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HSP60 monoclonal antibody (Mab11-13)

ADI-SPA-829-D 50 µg 251.00 USD
ADI-SPA-829-F 200 µg 513.00 USD
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Product Details

Alternative Name:Chaperonin 60, CPN60, HspD1, heat shock protein 60
Immunogen:Recombinant human Hsp60.
UniProt ID:P10809
GenBank ID:M34664
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Dolphin, Drosophila, Fish, Guinea pig, Hamster, Monkey, Mosquito, Porcine, Rabbit, Snake
Applications:Flow Cytometry, IF, IHC (PS), WB
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~60kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
Regulatory Status:RUO - Research Use Only
HSP60 monoclonal antibody (Mab11-13) Immunohistochemistry
Immunohistochemistry analysis of human spleen tissue stained with HSP60, mAb (Mab11-13) at 5µg/ml.
HSP60 monoclonal antibody (Mab11-13) Western blot
Western Blot Analysis of ADI-SPA-829: Lane 1: HSP60 protein(Human)(recombinant) (ADI-NSP-540), Lane 2: HeLa (Heat Shocked) Cell Lysate (ADI-LYC-HL101), Lane 3: PC-12 (Heat Shocked) Cell Lysate (ADI-LYC-PC101), Lane 4: 3T3 (Heat Shocked) Cell Lysate (ADI-LYC-3T101), Lane 5: GPC-16 (Heat Shocked) Cell Lysate
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HSP60 monoclonal antibody (Mab11-13) Immunohistochemistry HSP60 monoclonal antibody (Mab11-13) Western blot

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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