Product Details
| Alternative Name: | Heat shock protein 70, Hsc70, Hsp73 |
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| Clone: | 1D9 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant spinach Hsc70 (Hsp73). |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Porcine, Plant
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| Applications: | IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~78kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSC70 (plant, ER, BiP): Lane 1: MW marker, Lane 2: Apple, Lane 3: Carrot, Lane 4: Spinach.
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Product Literature References
Arabidopsis AZI1 family proteins mediate signal mobilization for systemic defence priming: N.M. Cecchini, et al.; Nat. Commun.
6, 7658 (2015),
Application(s): Western Blot,
Abstract;
Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation: Y. Zhang, et al.; Cell. Res.
25, 864 (2015),
Application(s): Western Blot,
Abstract;
Full Text
AtCCX3 Is an Arabidopsis Endomembrane H(+)-Dependent K(+) Transporter: K. Hirschi, et al. ; Plant Physiol.
148, 1474 (2008),
Application(s): WB using porcine cell lysates,
Abstract;
The Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses and reveals interplay among vacuolar transporters: K.D. Hirschi, et al. ; Plant Cell
15, 347 (2003),
Application(s): WB using plant (arabidopsis, tobacco) samples,
Abstract;
Mechanism of cell death and disease resistance induction by transgenic expression of bacterio-opsin: E. Lam, et al. ; Plant J.
30, 499 (2002),
Application(s): WB using plant (tobacco) samples,
Abstract;
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