Product Details
| Clone: | P4G7-H11 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Native bovine Ubiquitin. |
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| UniProt ID: | P0CG53 |
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| Gene/Protein Identifier: | NP_776558 (RefSeq) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Hamster, Rabbit
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| Applications: | Flow Cytometry, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~6.5kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK
Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10µg/mL
Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10µg/ml.
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Product Literature References
Expression quantitative trait loci mapping identified PtrXB38 as a key hub gene in adventitious root development in Populus: T. Yao, et al.; New Phytol. (2023),
Abstract;
Antibody targeting of E3 ubiquitin ligases for receptor degradation: H. Marei, et al.; Nature
610, 182 (2022),
Abstract;
Lifespan differences between queens and workers are not explained by rates of molecular damage: E.R. Lucas, et al.; Exp. Gerontol.
92, 1 (2017),
Abstract;
Distinct types of translation termination generate substrates for ribosome-associated quality control: N. Shcherbik, et al.; Nucleic Acids Res.
44, 6840 (2016),
Application(s): Cell analysis of the modification of distinct ribosomal species,
Abstract;
Full Text
Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells: T. Fujie, et al.; Int. J. Mol. Sci.
17, 1381 (2016),
Application(s): Western blot analysis of protein expression,
Abstract;
Full Text
ERK-dependent threonine phosphorylation of EGF receptor modulates receptor downregulation and signaling: S. Frank, et al. ; Cell. Signal.
20, 2145 (2008),
Application(s): WB using hamster cell lysates,
Abstract;
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