Product Details
Clone: | CerCLIP.1 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of human CLIP. |
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UniProt ID: | P04233 |
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GenBank ID: | K01144 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human
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Applications: | Flow Cytometry, IP, WB
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Recommended Dilutions/Conditions: | Flow Cytometry (1:200) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | CLIP (class II-associated invariant chain peptide) is an important peptide in the MHC-II antigen processing pathway, formed by the enzymatic cleavage of amino acids 81-104 from the invariant chain. During MHC-II synthesis in the ER and transport to endocytic vesicles, the antigen binding site of MHC-II is obstructed by the presence of the invariant chain. As the MHC-II complex moves through the Golgi, the invariant chain is degraded, leaving the CLIP fragment in the MHC-II peptide binding groove. CLIP is eventually displaced by the loading of antigenic peptides, followed by presentation on the APC cell surface to CD4+ T-cells. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules: P. Cresswell, et al. ; J. Exp. Med.
184, 2153 (1996),
Application(s): Other using human samples,
Abstract;
HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading: P. Cresswell, et al. ; Cell
82, 155 (1995),
Application(s): Other using human samples,
Abstract;
Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells: P. Cresswell, et al. ; Immunity
1, 595 (1994),
Application(s): IP, Flow using human samples,
Abstract;