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Convenient - assay most samples without extraction
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Robust - fully validated in multiple complex sample matrices
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Results from up to 37 samples in duplicate per 96-well plate
The PGF2α high sensitivity EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 3 hours. Absorbance is read at 405 nm. Simply the most sensitive quantification fo PGF2α commercially available in an enzyme immunoassay. Assay more samples without extraction.
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Product Details
| Alternative Name: | Prostaglandin F2α |
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| Sensitivity: | 0.98 pg/ml (range 1.95 - 2,000 pg/ml) |
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| Assay Time: | Overnight + 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of PGF2α in culture supernatants, milk, plasma, serum, saliva, and urine from any species. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention: A. Rapacz-Leonard, et al.; Sci. Rep.
10, 2089 (2020),
Abstract;
Full Text
Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci.
77, 107 (2019),
Application(s): ELISA using culture supernatants,
Abstract;
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology
84, 457 (2015),
Application(s): ELISA using mare sample,
Abstract;
Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells: I. Kowalczyk-Zieba, et al.; Prostaglandins Other Lipid Mediat.
121, 218 (2015),
Application(s): PGF2α measurements,
Abstract;
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