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Homogeneous - mix-and-read format easily adaptable to HTS applications
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Sensitive - fluorescent detection system
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Fast - results in just 1 hour
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.
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Product Details
| Sensitivity: | (range 0- 4 U/ml) |
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| Applications: | Fluorescent detection, HTS
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| Application Notes: | For the measurement of catalase in cell lysates from any species. |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Reaction buffer concentrate, Detection Reagent, Horseradish peroxidase concentrate, Hydrogen peroxide, Catalase enzyme |
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| Scientific Background: | Catalase is an antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system and functions to alleviate oxidative damage by catalyzing metabolism of the peroxide substrate. Accumulation of H2O2 can result in cellular damage through oxidation of proteins, DNA, and lipids, and has been linked to numerous pathologies including inflammation, cancer, diabetes, Parkinson’s disease, and Alzheimer’s. |
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| UniProt ID: | P00432 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Evaluation of the Antioxidant Activity of Levetiracetam in a Temporal Lobe Epilepsy Model: I.I. Mejía, et al.; Biomedicines
11, 848 (2023),
Abstract;
Increased oxidative stress contributes to enhance brain amyloidogenesis and blunts energy metabolism in sucrose-fed rat: effect of AMPK activation: L. Camacho-Castillo, et al.; Sci. Rep.
11, 19547 (2021),
Abstract;
Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium: S. Probst, et al.; Arch. Toxicol.
95, 2719 (2021),
Abstract;
Bacillus subtilis CBR05 induces Vitamin B6 biosynthesis in tomato through the de novo pathway in contributing disease resistance against Xanthomonas campestris pv. Vesicatoria: M. Chandrasekaran, et al.; Sci. Rep.
9, 6495 (2019),
Abstract;
Full Text
The biological age linked to oxidative stress modifies breast cancer aggressiveness: M.D.M. Saez-Freire, et al.; Free Radic. Biol. Med.
120, 133 (2018),
Abstract;
Vitamin B6 biosynthetic genes expression and antioxidant enzyme properties in tomato against, Erwinia carotovora subsp. carotovora: M. Chandrasekaran, et al.; Int. J. Biol. Macromol.
116, 31 (2018),
Abstract;
A multi-trait systems approach reveals a response cascade to bleaching in corals: S.G. Gardner, et al.; BMC Biol.
15, 117 (2017),
Abstract;
Full Text
Exploration of scalp surface lipids reveals squalene peroxide as a potential actor in dandruff condition: R. Jourdain, et al.; Arch. Dermatol. Res.
308, 153 (2016),
Application(s): Determined catalase activity,
Abstract;
Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells: S.J. Choi, et al.; Int. J. Nanomedicine
10, 3217 (2015),
Application(s): Assay using human lung alveolar carcinoma epithelial cells,
Abstract;
Full Text
Cystine dimethylester loading promotes oxidative stress and a reduction in ATP independent of lysosomal cystine accumulation in a human proximal tubular epithelial cell line: R. Sumayao, et al.; Exp. Physiol.
98, 1505 (2013),
Abstract;
