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Jnk2 monoclonal antibody (12C5)

ADI-905-648-100 100 µg 446.00 USD
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Product Details

Alternative Name:c-Jun N-terminal kinase 2, SAP kinase 1a, SAPK1a, MAP kinase 9, MAPK9
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of Jnk2.
UniProt ID:P45984
Species reactivity:Human, Mouse
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~54kDa by Western blot.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The Stress-activated protein kinases alpha and gamma (SAPKalpha and gamma), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinases belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.
Regulatory Status:RUO - Research Use Only
Jnk2 monoclonal antibody (12C5) Immunohistochemistry
Immunohistochemistry analysis of human hepatoma tissue with Jnk2 mAb (12C5).
Jnk2 monoclonal antibody (12C5) Western blot
Western blot analysis of serum starved cancer cells A431 (1), A549 (2), SKOV3 (3), OVCAR5 (4), HaCaT (5), PC3 (6), HeLa (7), and HepG2 (8) probed with Jnk2 mAb (12C5).
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Jnk2 monoclonal antibody (12C5) Immunohistochemistry Jnk2 monoclonal antibody (12C5) Western blot

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