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15(S)-HETE ELISA kit

ADI-901-051 5x96 wells 1,823.00 USD
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Alternative size available: ADI-900-051 (1x96 wells)
  • Sensitive - very low detection limit
  • Easy - few reagent preparation steps save time in the lab
  • Trusted - highly cited in peer-reviewed publications
  • Reliable - reproducible results day-after-day and lot-after-lot
  • Save with bulk kit package!
The 15(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. Absorbance is read at 405 nm.

Product Details

Alternative Name:15(S)-hydroperoxy tetraenoic eicosatetraenoic acid
Sensitivity:69.21 pg/ml (range 78.1 - 20,000 pg/ml)
Assay Time:Overnight + 1 hour
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of 15(S)-HETE in culture supernatants, plasma, serum, and urine from any species.
Wavelength:405 nm
Species reactivity:Species independent
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
Shipping:Blue Ice
Contents:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

ACSL4 and the lipoxygenases 15/15B are pivotal for ferroptosis induced by iron and PUFA dyshomeostasis in dopaminergic neurons: H. Bouchaoui, et al.; Free Radic. Biol. Med. 195, 145 (2023), Abstract;
Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci. 284, 119925 (2021), Abstract;
12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest. 130, 4999 (2020), Abstract; Full Text
Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer: E.H. Sarsour, et al.; J. Biol. Chem. 295, 6946 (2020), Abstract; Full Text
Early proteome shift and serum bioactivity precede diesel exhaust-induced impairment of cardiovascular recovery in spontaneously hypertensive rats: L.C. Thompson, et al.; Sci. Rep. 9, 6885 (2019), Application(s): ELISA using rat serum, Abstract; Full Text
Programming of macrophages by UV-irradiated apoptotic cancer cells inhibits cancer progression and lung metastasis: Y.B. Kim, et al.; Cell. Mol. Immunol. 16, 851 (2019), Application(s): ELISA using culture supernatants, Abstract;
Mice lacking L-12/15-lipoxygenase show increased mortality during kindling despite demonstrating resistance to epileptogenesis: M.A. Kanzler, et al.; Epilepsia Open 3, 255 (2018), Application(s): Cell Culture Supernatant, Abstract; Full Text
The atheroprotective role of lipoxin A4 prevents oxLDL-induced apoptotic signaling in macrophages via JNK pathway: J. Mai, et al.; Atherosclerosis 278, 259 (2018), Application(s): ELISA using rabbit artery samples, Abstract;
Proliferation and differentiation of adipose tissue in prolonged lean and obese critically ill patients: C. Goosens, et al.; Intensive Care Med. Exp. 5, 16 (2017), Abstract; Full Text
12/15-Lipoxygenase metabolites of arachidonic acid activate PPARĪ³: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text
Effect of a low-fat fish oil diet on proinflammatory eicosanoids and cell-cycle progression score in men undergoing radical prostatectomy: C. Gallet, et al.; Cancer Prev. Res. (Phila) 7, 97 (2014), Abstract;
12/15-lipoxygenase contributes to platelet-derived growth factor-induced activation of signal transducer and activator of transcription 3: T. Blazevic, et al.; J. Biol. Chem. 288, 35592 (2013), Application(s): ELISA using culture supernatants, Abstract; Full Text
A novel pathway involving progesterone receptor, 12/15-lipoxygenase-derived eicosanoids, and peroxisome proliferator-activated receptor gamma regulates implantation in mice: Q. Li, et al.; J. Biol. Chem. 279, 11570 (2004), Sample(s) tested: Mouse uteri homogenates., Abstract;
15-Lipoxygenase-1 mediates cyclooxygenase-2 inhibitor-induced apoptosis in gastric cancer: J. Wu, et al.; Carcinogenesis 24, 243 (2003), Sample(s) tested: AGS and MGC803 cells, Abstract;
Differential effects of aspirin and misoprostol on 15-hydroxyeicosatetraenoic acid generation by leukocytes from aspirin-sensitive asthmatic patients: M.L. Kowalski, et al.; J. Allergy Clin. Immunol. 112, 505 (2003), Sample(s) tested: Human leukocyte supernatant, Abstract;
Nonsteroidal anti-inflammatory drugs induce apoptosis in esophageal cancer cells by restoring 15-lipoxygenase-1 expression: I. Shureiqi, et al.; Cancer Res. 61, 4879 (2001), Sample(s) tested: Human esophageal cancer cells, Abstract;
15(S)-HETE modulates LTB(4) production and neutrophil chemotaxis in chronic bronchitis: M. Profita, et al.; Am. J. Physiol. Cell Physiol. 279, C1249 (2000), Sample(s) tested: Human induced sputum, Abstract;
15-Lipoxygenase-1 mediates nonsteroidal anti-inflammatory drug-induced apoptosis independently of cyclooxygenase-2 in colon cancer cells: I. Shureiqi, et al.; Cancer Res. 60, 6846 (2000), Sample(s) tested: Colorectal cancer cells (DLD-1), Abstract;
Differential metabolism of arachidonic acid in nasal polyp epithelial cells cultured from aspirin-sensitive and aspirin-tolerant patients: M.L. Kowalski, et al.; Am. J. Respir. Crit. Care Med. 161, 391 (2000), Sample(s) tested: Human nasal polyp epithelial cells, Abstract;
Normal high density lipoprotein inhibits three steps in the formation of mildly oxidized low density lipoprotein: step 1: M. Navab, et al.; J. Lipid Res. 41, 1481 (2000), Sample(s) tested: Human low density lipoprotein from serum, Abstract;
Hepatocytes are a rich source of novel aspirin-triggered 15-epi-lipoxin A(4): E. Titos, et al.; Am. J. Physiol. Cell Physiol. 277, C870 (1999), Sample(s) tested: Rat hepatocytes and CC-1 cells supernatant, Abstract;
Changes in expression of 15-lipoxygenase and prostaglandin-H synthase during differentiation of human tracheobronchial epithelial cells: E.M. Hill, et al.; Am. J. Respir. Cell Mol. Biol. 18, 662 (1998), Sample(s) tested: Human tracheobronchial epithelial cells, Abstract;

General Literature References

Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid: L.E. Rhodes, et al.; Br. J. Nutr. 28, 1 (2013), Abstract;

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