Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

8-iso-PGF ELISA kit

ADI-901-010 5x96 wells 1,900.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-010 (1x96 wells)
  • Fast - results from up to 36 samples in duplicate in less than 3 hours
  • Ready-to-use - liquid color-coded reagents and pre-coated plates reduce errors
  • Trusted - published in peer-reviewed journals
  • Reliable - reproducible results day-after-day and lot-after-lot
  • Save with bulk kit package!
The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F in biological fluids such as urine and in culture supernatants. To measure 8-iso-Prostaglandin F levels in serum, plasma or other tissue samples, use the Direct 8-iso-PGF EIA Kit.

8-iso-PGF is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF has been shown to circulate in plasma and is excreted in urine.

Product Details

Alternative Name:8-iso-Prostaglandin F2α
Sensitivity:16.3 pg/ml (range 6.1 - 100,000 pg/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of 8-iso-PGF in culture supernatants, tissue, and urine from any species. Cited sample types include plasma.
Wavelength:405 nm
Species reactivity:Species independent
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal

Product Literature References

Discriminating Different Bladder and Bladder Outlet Dysfunctions by Urinary Biomarkers in Women with Frequency–Urgency Syndrome: J.F. Jhang, et al.; Biomedicines 11, 673 (2023), Abstract;
Investigating Urine Biomarkers in Detrusor Underactivity and Detrusor Overactivity with Detrusor Underactivity Patients: Y.-H. Jiang, et al.; Biomedicines 11, 1191 (2023), Application(s): Human urine, Abstract; Full Text
Urinary Oxidative Stress Biomarker Levels Might Be Useful in Identifying Functional Bladder Disorders in Women with Frequency and Urgency Syndrome: Y.H. Jiang, et al.; J. Clin. Med. 12, 2336 (2023), Abstract;
Combustion-Derived Particulate PAHs Associated with Small Airway Dysfunction in Elderly Patients with COPD: T. Wang, et al.; Environ. Sci. Technol. 56, 10868 (2022), Abstract;
Role of urinary H2O2, 8-iso-PGF2α, and serum oxLDL/β2GP1 complex in the diabetic kidney disease: R. Sauriasari, et al.; PLoS One 17, e0263113 (2022), Abstract;
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat. 155, 106565 (2021), Abstract;
Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs: C. D'Agrosa, et al.; Respir. Res. 22, 196 (2021), Abstract;
Endothelin-1-Induced Microvascular ROS and Contractility in Angiotensin-II-Infused Mice Depend on COX and TP Receptors: C.S. Wilcox, et al.; Antioxidants (Basel) 8, E193 (2019), Abstract; Full Text
Effects of remote ischemic preconditioning and topical hypothermia in renal ischemia-reperfusion injury in rats: G.L. Motta, et al.; Acta Circ. Bras. 33, 396 (2018), Abstract;
NRF2 prevents hypertension, increased ADMA, microvascular oxidative stress, and dysfunction in mice with two weeks of ANG II infusion: C. Wang, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 314, R399 (2018), Application(s): ELISA of mouse urine, Abstract; Full Text
The correlation between urinary 8-iso-prostaglandin F2α and hydrogen peroxide toward renal function in T2DM patients consuming sulfonylurea and combination of metformin-sulfonylurea: R. Sauriasari, et al.; Curr. Diabetes Rev. 14, 189 (2018), Application(s): ELISA using human serum, Abstract;
Cardiorespiratory responses of air filtration: A randomized crossover intervention trial in seniors living in Beijing: Beijing Indoor Air Purifier StudY, BIAPSY: D. Shao, et al.; Sci. Total Environ. 603-604, 541 (2017), Application(s): Human blood plasma, Abstract;
Effects of a multimodal rehabilitation programme on inflammation and oxidative stress in oesophageal cancer survivors: the ReStOre feasibility study: E.M. Guinan, et al.; Support. Care Cancer 25, 749 (2017), Application(s): Neat hydrolysed human blood serum, Abstract;
Circulating Retinoic Acid Levels and the Development of Metabolic Syndrome: Y. Liu, et al.; J. Clin. Endocrinol. Metab. 101, 1686 (2016), Application(s): Human Plasma, Abstract;
Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung: M. Horie, et al.; Antioxidants (Basel) 5, 4 (2016), Application(s): Rat lung tissue lysates, Abstract; Full Text
Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids 115, 41 (2016), Application(s): Urine, Abstract; Full Text
Cellular and Soluble Inflammatory Markers in Induced Sputum of Composting Plant Workers: M. Raulf, et al.; Adv. Exp. Med. Biol. 858, 19 (2015), Abstract;
Moderate oral supplementation with docosahexaenoic acid improves platelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost. 114, 289 (2015), Application(s): Human Urine, Abstract;
Associations of retinol-binding protein 4 with oxidative stress, inflammatory markers, and metabolic syndrome in a middle-aged and elderly Chinese population: Y. Liu, et al.; Diabetol. Metab. Syndr. 6, 25 (2014), Application(s): Human plasma, Abstract; Full Text
Blood and urine 8-iso-PGF2α levels in babies of different gestational ages: S. Li, et al.; Int. J. Clin. Exp. Med. 7, 5477 (2014), Application(s): ELISA, Abstract; Full Text
Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats: D.B. Williams, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 302, R49 (2012), Application(s): ELISA using rat muscles, Abstract; Full Text
Increased plasma levels of 8-iso-PGF(2α) and IL-6 in an elderly population with depression: N. Dimopoulos, et al.; Psychiatry Research 161, 59 (2008), (ref. 1), Abstract;
Altered kidney graft high-energy phosphate metabolism in kidney-transplanted end-stage renal disease type 1 diabetic patients - A cross-sectional analysis of the effect of kidney alone and kidney-pancreas transplantation: P. Fiorina, et al. ; Diabetes Care 30, 597 (2007), Application(s): EIA using human urine, Abstract;
Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K. Lee, et al.; J. Med. Chem. 50, 1380 (2007), Application(s): EIA using rat & human whole blood, Abstract;
Role of the Src family kinase Lyn in TxA2 production, adenosine diphosphate secretion, Akt phosphorylation, and irreversible aggregation in platelets stimulated with gamma-thrombin: M.J. Cho, et al. ; Blood 99, 2442 (2002), Application(s): EIA using mouse platelets, Abstract;
Cyclooxygenase-1 and -2-Dependent Prostacyclin Formation in Patients With Atherosclerosis: O. Belton, et al.; Circulation 102, 840 (2000), Application(s): EIA using human serum, Abstract;
Sevoflurane and Isoflurane Do Not Enhance the Pre- and Postischemic Eicosanoid Production in Guinea Pig Hearts : B. Heindl, et al. ; Anesth. Analg. 90, 17 (2000), Application(s): EIA using guinea pig coronary effluent, Abstract;
A Sequence within the Cytoplasmic Tail of GpIIb Independently Activates Platelet Aggregation and Thromboxane Synthesis: G. Stephens, et al. ; J. Biol. Chem. 273, 20317 (1998), Application(s): EIA using human cell lysates, Abstract;

Related Products

8-iso-PGF ELISA kit 

ELISA, Colorimetric detection | Print as PDF
ADI-900-010 96 wells 474.00 USD
Do you need bulk/larger quantities?

Absorbance 96 Plate Reader 

Personal, Compact Plate Reader
ELISA, Colorimetric detection | Print as PDF
ENZ-INS-A96 1 ea 5,174.70 USD

Related Literature

Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Download as PDF

Immunoassay Kits
Immunoassay Kits
Download as PDF

All new literature pieces