- Fast - results from up to 36 samples in duplicate in less than 3 hours
- Ready-to-use - liquid color-coded reagents and pre-coated plates reduce errors
- Trusted - published in peer-reviewed journals
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The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. This is a colorimetric competitive immunoassay kit for the quantitative determination of free 8-iso-Prostaglandin F2α in biological fluids such as urine and in culture supernatants. To measure 8-iso-Prostaglandin F2α levels in serum, plasma or other tissue samples, use the Direct 8-iso-PGF2α EIA Kit.
8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.
Product Details
| Alternative Name: | 8-iso-Prostaglandin F2α |
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| Sensitivity: | 16.3 pg/ml (range 6.1 - 100,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of 8-iso-PGF2α in culture supernatants, tissue, and urine from any species. Cited sample types include plasma. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Combustion-Derived Particulate PAHs Associated with Small Airway Dysfunction in Elderly Patients with COPD: T. Wang, et al.; Environ. Sci. Technol.
56, 10868 (2022),
Abstract;
Role of urinary H2O2, 8-iso-PGF2α, and serum oxLDL/β2GP1 complex in the diabetic kidney disease: R. Sauriasari, et al.; PLoS One
17, e0263113 (2022),
Abstract;
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat.
155, 106565 (2021),
Abstract;
Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs: C. D'Agrosa, et al.; Respir. Res.
22, 196 (2021),
Abstract;
Endothelin-1-Induced Microvascular ROS and Contractility in Angiotensin-II-Infused Mice Depend on COX and TP Receptors: C.S. Wilcox, et al.; Antioxidants (Basel)
8, E193 (2019),
Abstract;
Full Text
Effects of remote ischemic preconditioning and topical hypothermia in renal ischemia-reperfusion injury in rats: G.L. Motta, et al.; Acta Circ. Bras.
33, 396 (2018),
Abstract;
NRF2 prevents hypertension, increased ADMA, microvascular oxidative stress, and dysfunction in mice with two weeks of ANG II infusion: C. Wang, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
314, R399 (2018),
Application(s): ELISA of mouse urine,
Abstract;
Full Text
The correlation between urinary 8-iso-prostaglandin F2α and hydrogen peroxide toward renal function in T2DM patients consuming sulfonylurea and combination of metformin-sulfonylurea: R. Sauriasari, et al.; Curr. Diabetes Rev.
14, 189 (2018),
Application(s): ELISA using human serum,
Abstract;
Cardiorespiratory responses of air filtration: A randomized crossover intervention trial in seniors living in Beijing: Beijing Indoor Air Purifier StudY, BIAPSY: D. Shao, et al.; Sci. Total Environ.
603-604, 541 (2017),
Application(s): Human blood plasma,
Abstract;
Effects of a multimodal rehabilitation programme on inflammation and oxidative stress in oesophageal cancer survivors: the ReStOre feasibility study: E.M. Guinan, et al.; Support. Care Cancer
25, 749 (2017),
Application(s): Neat hydrolysed human blood serum,
Abstract;
Circulating Retinoic Acid Levels and the Development of Metabolic Syndrome: Y. Liu, et al.; J. Clin. Endocrinol. Metab.
101, 1686 (2016),
Application(s): Human Plasma,
Abstract;
Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung: M. Horie, et al.; Antioxidants (Basel)
5, 4 (2016),
Application(s): Rat lung tissue lysates,
Abstract;
Full Text
Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids
115, 41 (2016),
Application(s): Urine,
Abstract;
Full Text
Cellular and Soluble Inflammatory Markers in Induced Sputum of Composting Plant Workers: M. Raulf, et al.; Adv. Exp. Med. Biol.
858, 19 (2015),
Abstract;
Moderate oral supplementation with docosahexaenoic acid improves
platelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost.
114, 289 (2015),
Application(s): Human Urine,
Abstract;
Associations of retinol-binding protein 4 with oxidative stress, inflammatory markers, and metabolic syndrome in a middle-aged and elderly Chinese population: Y. Liu, et al.; Diabetol. Metab. Syndr.
6, 25 (2014),
Application(s): Human plasma,
Abstract;
Full Text
Blood and urine 8-iso-PGF2α levels in babies of different gestational ages: S. Li, et al.; Int. J. Clin. Exp. Med.
7, 5477 (2014),
Application(s): ELISA,
Abstract;
Full Text
Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats: D.B. Williams, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
302, R49 (2012),
Application(s): ELISA using rat muscles,
Abstract;
Full Text
Increased plasma levels of 8-iso-PGF(2α) and IL-6 in an elderly population with depression: N. Dimopoulos, et al.; Psychiatry Research
161, 59 (2008), (ref. 1),
Abstract;
Altered kidney graft high-energy phosphate metabolism in kidney-transplanted end-stage renal disease type 1 diabetic patients - A cross-sectional analysis of the effect of kidney alone and kidney-pancreas transplantation: P. Fiorina, et al. ; Diabetes Care
30, 597 (2007), Application(s): EIA using human urine,
Abstract;
Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K. Lee, et al.; J. Med. Chem.
50, 1380 (2007),
Application(s): EIA using rat & human whole blood,
Abstract;
Role of the Src family kinase Lyn in TxA2 production, adenosine diphosphate secretion, Akt phosphorylation, and irreversible aggregation in platelets stimulated with gamma-thrombin: M.J. Cho, et al. ; Blood
99, 2442 (2002), Application(s): EIA using mouse platelets,
Abstract;
Cyclooxygenase-1 and -2-Dependent Prostacyclin Formation in Patients With Atherosclerosis: O. Belton, et al.; Circulation
102, 840 (2000),
Application(s): EIA using human serum,
Abstract;
Sevoflurane and Isoflurane Do Not Enhance the Pre- and Postischemic Eicosanoid Production in Guinea Pig Hearts : B. Heindl, et al. ; Anesth. Analg.
90, 17 (2000),
Application(s): EIA using guinea pig coronary effluent,
Abstract;
A Sequence within the Cytoplasmic Tail of GpIIb Independently Activates Platelet Aggregation and Thromboxane Synthesis: G. Stephens, et al. ; J. Biol. Chem.
273, 20317 (1998), Application(s): EIA using human cell lysates,
Abstract;
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