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Efficient - only 50µl sample volume required
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Ultrasensitive - detect <80 pg/ml
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Higher throughput - assay up to 37 samples in duplicate per 96 well kit
The Gastrin I (rat), EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Ready-to-use liquid color-coded reagents are provided to reduce error in the lab. This kit utilizes low sample volume and the ability to measure up to 37 samples in duplicate per 96-well plate.
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Product Details
| Sensitivity: | 78.1 pg/ml (range 78.1 - 5,000 pg/ml) |
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| Assay Time: | 5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of rat Gastrin I in culture supernatants, plasma, and serum. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Rat
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| Crossreactivity: | Rat Gastrin I (100%), Cholecystokinin 26-33 (CCK-8) (100%), human Gastrin I (30%), mouse Gastrin I (20.5%), Minigastrin (G13-I) (11%), Gastrin Tetrapeptide (CCK-4) (0.75%), Glucagon (0.5%) and <0.2%: Gastrin Inhibitory Polypeptide (GIP), Bombesin, Somatostatin-14, Gastrin Releasing Peptide (GRP) |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G cells of the antrum mucosa, and are potent stimulators of gastric acid secretion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. |
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| UniProt ID: | P04563 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Role of gastric acid inhibition, prostaglandins and endogenous-free thiol groups on the gastroprotective effect of a proteolytic fraction from Vasconcellea cundinamarcensis latex: A.C. Araujo e Silva, et al.; J. Pharm. Pharmacol.
67, 133 (2015),
Abstract;
Low molecular weight fucoidan ameliorating the chronic cisplatin-induced delayed gastrointestinal motility in rats: M.Y. Song, et al.; Food Chem. Toxicol.
50, 4468 (2012),
Abstract;
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