- Time savings - determine the isotype and concentration of monoclonal antibodies
- Quantitative - suitable in determining concentration
- Specific - no reactivity with other immunoglobulins
- Fast - results from up to 40 samples in duplicate in just 90 minutes
The IgM (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 90 minutes. Absorbance is read at 450 nm. Ideal for determining the isotype and concentration of monoclonal antibodies.
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Product Details
| Sensitivity: | 0.60 ng/ml (range 3.91 - 250 ng/ml) |
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| Assay Time: | 90 minutes |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of IgM in ascites fluid, culture supernatants, and serum of mouse origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse
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| Crossreactivity: | Mouse IgM (100%), rat IgM (60%), human IgM (<0.4%), mouse IgG1 (<0.2%) and <0.10%: mouse IgG2a, mouse IgG2b, mouse IgG3 |
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | GxM IgG Microtiter plate, mouse IgM conjugate, Assay buffer 13 concentrate, Wash buffer concentrate, mouse IgGM standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Trypanocidal effects of cisplatin alone and in combination with Nigella sativa oil on experimentally infected mice with Trypanosoma evansi: N.A.E. Nassef, et al.; Iran. J. Parasitol.
13, 89 (2018),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
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