Replaces Prod. #: ADI-900-113
- Time savings - determine the isotype and concentration of monoclonal antibodies
- Quantitative - suitable in determining concentration
- Specific - no reactivity with other immunoglobulins
- Fast - results from up to 40 samples in duplicate in just 90 min
The IgG2a (mouse) ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in 90 minutes. Ideal for determining the isotype and concentration of monoclonal antibodies.
Typical standard curve for IgG2a (mouse) ELISA Kit (ADI-900-113A)
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Product Details
| Sensitivity: | 47.3 pg/ml (range 7.81 - 250 ng/ml) |
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| Assay Time: | 90 minutes |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of IgG2a in culture supernatants and serum of mouse origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse
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| Crossreactivity: | Mouse IgG2a (100%), mouse IgG3 (0.49%), mouse IgM (0.12%) and <0.01%: rat IgG2a, mouse IgG1, mouse IgG2b |
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | GxM IgG Microtiter plate, mouse IgG2a conjugate, Assay buffer 13 concentrate, Wash buffer concentrate, mouse IgG2a standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (γ) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. We offer isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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