[pThr¹⁸³/Tyr¹⁸⁵]Jnk1/2 ELISA kit



ADI-900-106	96 wells	643.00 USD
Do you need bulk/larger quantities?

Sensitive - use less precious sample per test compared to Western blot
Higher throughput - test up to 40 samples in duplicate in just 3 hours
Quantitative - obtain statistically signficant results and compare data between multiple experiments
Ready-to-use - save time and reduce errors with pre-coated plates

The [pThr¹⁸³/Tyr¹⁸⁵]Jnk1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

[pThr183/Tyr185]Jnk1/2 ELISA kit Kit graph

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Product Details

Alternative Name:	c-Jun N-terminal kinase 1/2, SAP kinase 1a/c, MAP kinase 8/9, MAPK8/9

Sensitivity:	75.8 pg/ml (range 125 - 4,000 pg/ml)

Assay Time:	3 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of [pThr¹⁸³/Tyr¹⁸⁵]Jnk1/2 in cell lysates of human, mouse, and rat origin.

Wavelength:	450 nm

Species reactivity:	Human, Mouse, Rat

Crossreactivity:	Phospho Jnk1 (100%), phospho Jnk2 (98.6%), phospho p38 (0.42%), phospho Erk2 (0.16%) and <0.01%: non-phosphorylated Erk2, non-phosphorylated Jnk1

Use/Stability:	Store all components at 4°C, except standard at -20°C.

Shipping:	Blue Ice

Contents:	Microtiter plate, Conjugate, Antibody, Assay buffer 4 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2

Scientific Background:	The Stress-activated protein kinases alpha and γ (SAPKα and γ), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinase belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.

UniProt ID:	P45983 (Jnk1), P45984 (Jnk2)

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity: G. Kravchenko, et al.; Nat. Prod. Bioprospect. 12, 30 (2022), Abstract;