[pThr¹⁸⁰/Tyr¹⁸²]p38 ELISA kit



ADI-900-101	96 wells	773.00 USD
Do you need bulk/larger quantities?

Sensitive - use less precious sample per test compared to Western blot
Higher throughput - test up to 40 samples in duplicate in just 3 hours
Quantitative - obtain statistically signficant results and compare data between multiple experiments
Ready-to-use - save time and reduce errors with pre-coated plates

The [pThr¹⁸⁰/Tyr¹⁸²]p38 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Use less precious sample per test compared to Western blot with higher throughput sample analysis; measure up to 40 samples in duplicate per kit.

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Product Details

Alternative Name:	SAPK2, SAP kinase 2

Sensitivity:	52.1 pg/ml (range 156 - 5,000 pg/ml)

Assay Time:	3 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of [pThr¹⁸⁰/Tyr¹⁸²]p38 in cell lysates of human and mouse origin.

Wavelength:	450 nm

Species reactivity:	Human, Mouse

Crossreactivity:	Phospho p38 (100%) and <0.01%: non-phosphorylated p38, phospho Jnk, non-phosphorylated Jnk, AKT, non-phosphorylated Erk, phospho Erk

Use/Stability:	Store all components at 4°C, except standard at -20°C.

Shipping:	Dry Ice and Blue Ice

Contents:	Microtiter plate, Conjugate, Antibody, Assay buffer 4 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2

Scientific Background:	Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38α (SAPK2, CSBP) and its splice variant Mxi; p38β and β2; p38γ (ERK6, SAPK3); and p38δ (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.

UniProt ID:	Q16539 (human p38α), Q15759 (human p38β), P53778 (p38γ), O15264 (p38δ)

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway: S. Sunilkumar, et al.; Am. J. Physiol. Cell Physiol. 316, C913 (2019), Abstract;

Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models: K.C. Choi, et al. ; J. Ethnopharmacol. 179, 55 (2016), Application(s): Measurement of p-p38 kinase in rat whole cell lysates, Abstract;

Catechin-7-O-β-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats: S.H. Kook, et al.; Int. Immunopharmacol. 29, 521 (2015), Application(s): ELISA measuring enzymatic activity of p38 kinase in rat tissue, Abstract;