- Results in <3 hours
- Sensitive to 1.01 pg/mL
- Measure up to 36 samples per 96 well plate
- Ready-to-use reagents and pre-coated plates reduce errors and save your time
The IL-6 (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours.
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Product Details
| Alternative Name: | Interleukin-6 |
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| Sensitivity: | 1.01 pg/ml (range 7.81-1,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of mouse Il-6 in culture supernatants and serum. Cited sample type includes tissue. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution |
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| Scientific Background: | Interleukin-6 (IL-6) is a cytokine critical to the regulation of the immune and hematopoietic systems. Human IL-6 is expressed by T-cells, mast cells, monocytes, macrophages, fibroblasts, endothelial cells, keratinocytes, and many tumor cell lines. It appears to take part in acute phase reactions and response to injury and inflammation. Human IL-6 also stimulates the differentiation of B-cells for antibody production, promotes expansion of activated T-cells, expands hematopoietic cell production, and induces the expression of acute phase proteins. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P08505 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Bcl-3 promotes TNF-induced hepatocyte apoptosis by regulating the deubiquitination of RIP1: Y. Hu, et al.; Cell Death Differ.
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Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res.
66, e2200082 (2022),
Abstract;
Serum biomarkers based neurotrauma severity scale: a study in the mice model of fluid percussion injury: M. Aleem, et al.; Acta Neurobiol. Exp. (Wars.)
82, 147 (2022),
Abstract;
Skin Dryness Induced in the KK-Ay/TaJcl Type 2 Diabetes Mouse Model Deteriorates Following Dapagliflozin Administration: T. Horikawa, et al.; Biol. Pharm. Bull.
45, 934 (2022),
Abstract;
Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts: Y. Sueda, et al.; Yonoago Acta Med.
65, 111 (2022),
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Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals
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Abstract;
4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci.
37, 604 (2021),
Application(s): IL-6 quantification in mouse blood,
Abstract;
BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res.
169, 105611 (2021),
Abstract;
Screening of Anti-Inflammatory Components of Qin Jin Hua Tan Tang by a Multivariate Statistical Analysis Approach for Spectrum-Effect Relationships: F. Duan, et al.; J. Anal. Methods Chem.
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Abstract;
Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H. U. Shih, et al.; J. Pers. Med.
11, 436 (2021),
Abstract;
A diet enriched with curcumin promotes resilience to chronic social defeat stress: A.V. Aubry, et al.; Neuropsychopharmacology
44, 733 (2019),
Abstract;
MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage: P. Sousa-Victor, et al.; Nat. Metab. 1, 276 (2019), Application(s): ELISA using tissue extracts,
Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth: S. Sancilio, et al.; Nanomaterials (Basel)
9, 1656 (2019),
Abstract;
Full Text
Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol.
358, 10 (2018),
Application(s): ELISA using supernatants prepared from tissue homogenates,
Abstract;
APC loss in breast cancer leads to doxorubicin resistance via STAT3 activation: M.K. VanKlompenberg , et al.; Oncotarget
8, 102868 (2017),
Abstract;
The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol.
18, 519 (2017),
Abstract;
Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol.
20, 415 (2016),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun.
478, 935 (2016),
Application(s): Measurement of cytokine levels in serum and cell culture medium,
Abstract;
Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus: M. Taniguchi, et al.; Sci. Rep.
6, 37829 (2016),
Abstract;
Full Text
The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother.
81, 111 (2016),
Application(s): ELISA using mouse blood samples,
Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol.
29, 344 (2015),
Application(s): ELISA using culture supernatants,
Abstract;
Mechanotransduction via TRPV4 regulates inflammation and differentiation in fetal mouse distal lung epithelial cells: P.S. Navak, et al.; Respir. Res.
16, 60 (2015),
Abstract;
Full Text
The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol.
31, 88 (2015),
Application(s): ELISA using cell culture supernatants,
Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep.
40, 269 (2013),
Application(s): EIA using culture supernatants from RAW264.7 macrophage cells,
Abstract;
Reduced IL-10 Production in Fetal Type II Epithelial Cells Exposed to Mechanical Stretch Is Mediated via Activation of IL-6-SOCS3 Signaling Pathway: M.A. Hokenson, et al.; PLoS One
8, e59598 (2013),
Abstract;
Full Text
Molecular mechanism of Jmjd3‐mediated interleukin‐6 gene regulation in endothelial cells underlying spinal cord injury: K. Lee, et al.; J. Neurochem.
122, 272 (2012),
Application(s): ELISA using culture supernatants,
Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum.
62, 732 (2010),
Application(s): EIA using mouse serum and cell culture supernatant,
Abstract;
Full Text
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol.
150, 332 (2007),
Application(s): EIA using mouse tissue homogenate,
Abstract;
Full Text
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