Alternative Name: | High affinity immunoglobulin γ Fc receptor I, IgG Fc Receptor I, IGFR1, FcγRIa |
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Clone: | AT37F7 |
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Host: | Mouse |
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Isotype: | IgG2bκ |
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Immunogen: | Recombinant human FCGR1A (aa 16-292) purified from E. coli. |
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UniProt ID: | P12314 |
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Source: | Purified from ascites. |
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Species reactivity: | Human
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Applications: | ELISA, Flow Cytometry, ICC, IF, WB
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Recommended Dilutions/Conditions: | Western Blot (1:2,000). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A affinity purified |
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Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | CD64 belongs to the immunoglobulin superfamily and it is a high-affinity Fc-gamma receptor. It plays a role in both innate and adaptive immune responses. |
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Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of ENZ-ABS725 in HeLa cell line, staining at 2-5µg for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Flow cytometry analysis of ENZ-ABS725 in Jurkat cell line, staining at 2-5µg for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
ICC/IF analysis of CD64 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FCGR1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
The recombinant protein (20µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD64 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.