Replaces Prod. #: ENZ-ABS112
Product Details
| Alternative Name: | β-O-linked N-acetylglucosamine |
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| Clone: | CTD110.6 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Synthetic peptide containing serine-O-GlcNAc. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. |
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| Crossreactivity: | Does not cross-react with peptide determinants or other closely-related carbohydrate antigens. |
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| Applications: | IF, IHC (PS)
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| Recommended Dilutions/Conditions: | For immunofluorescence microscopy, a concentration range of 2.5 - 10 μg/ml is recommended.
For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 10 µg/ml.
It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes: | Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker. |
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| Purity Detail: | Purified by affinity chromatography. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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