Fluo-3 is widely used in flow cytometry, confocal laserscanning microscopy and fluorescence spectroscopy. The probe has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Once inside the cell, Fluo-3 becomes fluorescent, in the presence of free Ca (II). Fluo-3 is a long wavelength calcium probe (488 nm excitable). The longer wavelength of absorption is convenient for minimizing photodamage in cells. Wavelength Maxima: Excitation 506nm, Emission 526nm
Product Details
Alternative Name: | 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid . 5NH3 |
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MW: | 854.7 |
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CAS: | 339221-91-9 |
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Purity: | ≥95% (HPLC) |
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Solubility: | Soluble in DMSO. |
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Shipping: | Ambient Temperature |
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Long Term Storage: | -20°C |
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Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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Handling: | Protect from light. Keep cool and dry. |
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Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux: S. Bailey & P.J. Macardle; J. Immunol. Methods
311, 220 (2006),
Abstract;
A laser scanning confocal microscopy method. Simultaneous detection of intracellular Ca2+ and apoptosis using Fluo-3 and Hoechst 33342: T. Zhang, et al.; Anal. Quant. Cytol. Histol.
22, 93 (2000),
Abstract;