Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.
Product Details
Alternative Name: | Biotin-17-adenosine-5’-triphosphate |
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Formula: | C34H57N10O17P3S |
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MW: | 1002.8 (free acid) |
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Concentration: | 10mM |
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Formulation: | Liquid. Solution in water. |
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Extinction Coefficient: | 18,000 M-1 cm-1 (267 nm, pH 7.0) |
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Purity: | ≥93% (HPLC) |
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Purity Detail: | Contains <5% Bio-17-ADP. |
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Appearance: | Clear, colorless liquid. |
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Shipping: | Dry Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. |
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Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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Regulatory Status: | RUO - Research Use Only |
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