NEDD8-AMC is a fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a particularly useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Details
| MW: | 8.735 kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | Q15843 |
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| Concentration: | 1 mg/ml |
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| Formulation: | Solution in DMSO. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Liquid. |
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| Application Notes: | Uses:
1. Substrate for deNEDDylating enzyme activity assays.
2. Identification/confirmation of enzyme deNEDDylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211). |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A widely distributed family of eukaryotic and bacterial deubiquitinases related to herpesviral large tegument proteins: I. Erven, et al.; Nat. Commun.
13, 7643 (2022),
Abstract;
A family of unconventional deubiquitinases with modular chain specificity determinants: T. Hermanns, et al.; Nat. Commun.
9, 799 (2018),
Abstract;
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