Fluorogenic substrate for deSUMOylating enzymes. SUMO1-AMC is a sensitive fluorogenic substrate for deSUMOylating enzymes, including SENP1 (Prod. No. BML-UW9760) and SENP2 (Prod. No. BML-UW9765). It is a particularly useful reagent for the study of deSUMOylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Details
| Alternative Name: | Sentrin-1-AMC |
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| MW: | ~11.3kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P63165 |
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| Formulation: | Liquid. In 50mM HEPES, pH 7.5, containing 100 mM NaCl. |
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| Purity: | ≥95% (HPLC) |
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| Application Notes: | Uses:
1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211). |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -80°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 12 months after receipt when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Technical Info/Product Notes: | Typical assay conditions: Assay substrate concentration: 10nM-1.0µM. Enzyme concentrations: 10nM-1µM. Release of AMC fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths). |
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| Regulatory Status: | RUO - Research Use Only |
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DeSUMOylation Assay: 1µM SENP1 CD, His
6-tagged (Prod. No.
BML-UW9760); 500nM SUMO-1-AMC (Prod. No. BML-UW0040); 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT."
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