Product Details
| Alternative Name: | Phosphatase of regenerating liver 1, Protein tyrosine phosphatase type IVA 1 |
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| MW: | ~19.8kDa |
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| Source: | Produced in E. coli. The catalytic domain of PRL-1 (aa 2-173) is fused at the N-terminus to a His-tag. |
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| EC: | 3.1.3.48 |
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| UniProt ID: | Q93096 |
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| Formulation: | Liquid. In 40mM TRIS-HCl, pH 8.0, containing 110mM sodium chloride, potassium chloride, 200mM imidazole, 20% glycerol and 3mM DTT. |
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| Purity: | ≥75% (SDS-PAGE) |
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| Specific Activity: | ≥0.168U/min/μg. One unit will hydrolyze 1pmol para-nitrophenyl phosphate (PNPP) per minute at 37°C. Assay buffer: 50mM TRIS, pH 7.4, containing 150mM sodium chloride, 5mM dithiothreitol and 12.5mM PNPP. |
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| Application Notes: | Useful for studies of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Dilution of the enzyme followed by refreezing may lead to loss of activity. |
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| Scientific Background: | PRL phosphatases comprise a class of small oncogenic phosphatases that are prenylated at their carboxyl-termini. PRL-1 is overexpressed during cell proliferation in the liver and during differentiation of epithelial cells in the digestive tract. It augments MMP-2 and MMP-9 expression, which in turn induce cell migration and invasion. |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
PRL1 Promotes Cell Migration and Invasion by Increasing MMP2 and MMP9 Expression through Src and ERK1/2 Pathways (dagger): Y. Luo, et al.; Biochemistry
48, 1838 (2009),
Abstract;
Expression of PRL-1 nuclear PTPase is associated with proliferation in liver but with differentiation in intestine: R.H. Diamond, et al.; Am. J. Physiol.
271, G121 (1996),
Abstract;
PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth: R.H. Diamond, et al.; Mol. Cell. Biol.
14, 3752 (1994),
Abstract;
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