Product Details
| Alternative Name: | Protein tyrosine phosphatase 1B |
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| MW: | ~37.4kDa |
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| Source: | Produced in E. coli. Human PTP1B (protein tyrosine phosphatase 1B) (aa 1-322). |
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| UniProt ID: | P18031 |
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| Formulation: | Liquid. In 50mM HEPES, pH7.2, containing 1mM EDTA, 5mM DTT, and 0.05% NP-40. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Specific Activity: | ≥20 µmol/min/mg at 30°C using 0.15mM of phosphopeptide substrate based on EGFR residues 988-998 (Prod. No. BML-P323) in 50mM HEPES, pH 7.2, 1mM EDTA, 1mM DTT, 0.05% NP-40, and 0.2µg/µl BSA. |
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| Application Notes: | Useful for the study of tyrosine phosphatase kinetics, substrate specificity and for screening inhibitors. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | PTP1B is the ubiquitous, prototypical nontransmembrane PTP originally identified in human placenta. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg, Lane 4: 5 µg PTP1B. The higher molecular weight bands are BSA included for stability in storage buffer.
Western Blot analysis of PTP1B (human), (recombinant) (Prod. No. BML-SE332): Lane 1: MW Marker, Lane 2: 100ng of PTP1B (human), (recombinant) probed with Anti-6X His tag® antibody.
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Product Literature References
Effects of KY-903, a Novel Tetrazole-Based Peroxisome Proliferator-Activated Receptor γ Modulator, in Male Diabetic Mice and Female Ovariectomized Rats: Y. Ito, et al.; Biol. Pharm. Bull.
44, 659 (2021),
Abstract;
PTP1B inhibition studies of biological active phloroglucinols from the rhizomes of Dryopteris crassirhizoma: Kinetic properties and molecular docking simulation: N.V. Phong, et al.; Int. J. Biol. Macromol.
1889, 719 (2021),
Abstract;
Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem.
85, 505 (2019),
Application(s): Activity assay,
Abstract;
Absolute Structures of Wedelolide Derivatives and Structure-Activity Relationships of Protein Tyrosine Phosphatase 1B Inhibitory ent-Kaurene Diterpenes from Aerial Parts of Wedelia spp. Collected in Indonesia and Japan: D.B. Abdjul, et al.; Chem. Pharm. Bull. (Tokyo)
66, 682 (2018),
Abstract;
Full Text
Cladosporamide A, a new protein tyrosine phosphatase 1B inhibitor, produced by an Indonesian marine sponge-derived Cladosporium sp: H. Rotinsulu, et al.; J. Nat. Med.
72, 779 (2018),
Abstract;
High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Miconia albicans: R. de Cassia Lemos Lima, et al.; Molecules
23, E1755 (2018),
Abstract;
Full Text
Quadruple high-resolution α-glucosidase/α-amylase/PTP1B/radical scavenging profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude root bark of Morus alba L: Y. Zhao, et al.; J. Chromatogr. A
1556, 55 (2018),
Abstract;
Protein tyrosine phosphatase 1B inhibitory properties of seco-cucurbitane triterpenes obtained from fruiting bodies of Russula lepida: W. Maarisit, et al.; J. Nat. Med.
71, 334 (2017),
Abstract;
Prunin is a highly potent flavonoid from Prunus davidiana stems that inhibits protein tyrosine phosphatase 1B and stimulates glucose uptake in insulin-resistant HepG2 cells: H.A. Jung, et al.; Arch. Pharm. Res.
40, 37 (2017),
Abstract;
PTP1B inhibitors from the seeds of Iris sanguinea and their insulin mimetic activities via AMPK and ACC phosphorylation: J.L. Yang, et al.; Bioorg. Med. Chem. Lett.
27, 5076 (2017),
Abstract;
Coumarins from Angelica decursiva inhibit α-glucosidase activity and protein tyrosine phosphatase 1B: M.Y. Ali, et al.; Chem. Biol. Interact.
252, 93 (2016),
Application(s): Used in treatment of Diabetes mellitus,
Abstract;
High-resolution PTP1B inhibition profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy: Proof-of-concept and antidiabetic constituents in crude extract of Eremophila lucida: Y. Tahtah, et al.; Fitoterapia
110, 52 (2016),
Application(s): PTP1B enzyme was used for the microplate-based PTP1B inhibition assay,
Abstract;
Identification and evaluation of magnolol and chrysophanol as the principle protein tyrosine phosphatase-1B inhibitory compounds in a Kampo medicine, Masiningan: T. Onoda, et al.; J. Ethnopharmacol.
186, 84 (2016),
Application(s): PTP1B inhibitory activity,
Abstract;
Isopetrosynol, a New Protein Tyrosine Phosphatase 1B Inhibitor, from the Marine Sponge Halichondria cf. panicea Collected at Iriomote Island: D.B. Abdjul, et al.; Chem. Pharm. Bull.
64, 733 (2016),
Application(s): Protein - used with inhibitory assay and assessing biological activity,
Abstract;
Full Text
Molecular Modeling of Licochalcone E as Protein Tyrosine Phosphatase 1B Inhibitor: E. Kim, et al.; Bull. Korean Chem. Soc. 37, 2102 (2016),
Protein tyrosine phosphatase 1B and α-glucosidase inhibitory activities of Pueraria lobata root and its constituents: S.H. Seong, et al.; J. Ethnopharmacol.
194, 706 (2016),
Application(s): Inhibitory activity and enzyme kinetic analysis, coral,
Abstract;
Structures and biological activities of triterpenes and sesquiterpenes obtained from Russula lepida: J.S. Lee, et al.; Phytochemistry
127, 63 (2016),
Application(s): Fluorescence microscopy assay,
Abstract;
The potent inhibitors of protein tyrosine phosphatase 1B from the fruits of Melaleuca leucadendron: A. Saifudin, et al.; Pharmacognosy Res.
8, S38 (2016),
Application(s): Protein tyrosine phosphatase 1B inhibitory activity,
Abstract;
Full Text
Antihyperglycemic and antioxidant activities of twig extract from Cinnamomum osmophloeum: G.M. Lin, et al.; J. Tradit. Complement. Med.
6, 281 (2015),
Application(s): Assay,
Abstract;
Full Text
Protein tyrosine phosphatase 1B inhibitory activity of alkaloids from Rhizoma Coptidis and their molecular docking studies: J.S. Choi, et al.; J. Ethnopharmacol.
171, 28 (2015),
Application(s): Inhibition Assay,
Abstract;
Selaginellin and biflavonoids as protein tyrosine phosphatase 1B inhibitors from Selaginella tamariscina and their glucose uptake stimulatory effects: P. H. Nguyen, et al.; Bioorg. Med. Chem.
23, 3730 (2015),
Application(s): Assay,
Abstract;
Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from the marine-derived fungus Trichoderma sp: H. Yamazaki, et al.; J. Antibiot. (Tokyo)
68, 628 (2015),
Application(s): Inhibitory Assay,
Abstract;
Two new diphenyl ethers from Acanthopanax senticosus (Rupr. & Maxim.) Harms with PTP1B inhibitory activity: N. Li, et al.; Phytochem. Lett. 13, 286 (2015),
Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae): S.H. Tiong, et al.; Fitoterapia
102, 182 (2015),
Application(s): Assay,
Abstract;
Bioactive compounds from culinary herbs inhibit a molecular target for type 2 diabetes management, dipeptidyl peptidase IV: A.M. Bower, et al.; J. Agric. Food Chem.
62, 6147 (2014),
Abstract;
Conjugation-free, visual, and quantitative evaluation of inhibitors on protein tyrosine kinases and phosphatases with a luminescent Tb(III) complex: H. Akiba, et al.; Anal. Bioanal. Chem.
406, 2957 (2014),
Abstract;
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.)
50, 10875 (2014),
Abstract;
Development of cell-active non-peptidyl inhibitors of cysteine cathepsins: D. Dana, et al.; Bioorg. Med. Chem.
21, 2975 (2013),
Abstract;
Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design: Y.A. Puius, et al.; PNAS
94, 13420 (1997),
Abstract;
Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas): F. Liu, et al.; J. Biol. Chem.
271, 31290 (1996),
Abstract;
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