Irreversible, cell-permeable inhibitor of granzyme B (ID50=0.3 µM). Does not inhibit granzyme A. Inhibition of granzyme B with Z-AAD-CMK prevented caspase-3 activation in T cells.
Product Details
| Alternative Name: | Granzyme B inhibitor |
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| Sequence: | Benzyloxycarbonyl-Ala-Ala-Asp-chloromethylketone |
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| Formula: | C19H24ClN3O7 |
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| MW: | 441.9 |
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| Source: | Synthetic. |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in DMSO (25mg/ml), 100% ethanol (25mg/ml) or water (20mg/ml). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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| Handling: | After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer: M. Kim, et al.; PLoS One
13, e0198347 (2018),
Abstract;
Full Text
Granzyme B expression is enhanced in human monocytes by TLR8 agonists and contributes to antibody-dependent cellular cytotoxicity: S. Elavazhagan, et al.; J. Immunol.
194, 2786 (2015),
Abstract;
Full Text
Cytotoxic T cells induce proliferation of chronic myeloid leukemia stem cells by secreting interferon-γ: C. Schürch, et al.; J. Exp. Med.
210, 605 (2013),
Abstract;
Full Text
IFN-γ stimulated human umbilical-tissue-derived cells potently suppress NK activation and resist NK-mediated cytotoxicity in vitro: C. Noone, et al.; Stem Cells Dev.
22, 3003 (2013),
Abstract;
Full Text
IL-21–stimulated human plasmacytoid dendritic cells secrete granzyme B, which impairs their capacity to induce T-cell proliferation: J.J. Karrich, et al.; Blood.
121, 3103 (2013),
Abstract;
Full Text
Tumor-reactive CD4+ T cells develop cytotoxic activity and eradicate large established melanoma after transfer into lymphopenic hosts: S.A. Quezada, et al.; J. Exp. Med.
207, 637 (2010),
Abstract;
Full Text
General Literature References
Selective inhibition of dipeptidyl peptidase I, not caspases, prevents the partial processing of procaspase-3 in CD3-activated human CD8(+) T lymphocytes.: N. Bidere et al.; J. Biol. Chem.
277, 32339 (2002),
Abstract;
Granzymes (lymphocyte serine proteases): characterization with natural and synthetic substrates and inhibitors.: C.M. Kam et al.; Biochem. Biophys. Acta
1477, 301 (2000),
Abstract;
