Product Details
| Alternative Name: | Leucine-rich repeat-containing protein 32, GARP, Garpin, Glycoprotein A repetitions predominant |
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| Clone: | Plato-1 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Recombinant human LRRC32 (aa 20-627). |
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| UniProt ID: | Q14392 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Mouse
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| Applications: | Flow Cytometry
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Keep conjugated formats at +4°C. Do not freeze. Protect from light. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Scientific Background: | LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis, intracellular staining, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (FITC conjugate) at a concentration of 50μg/ml.
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Product Literature References
Feline glycoprotein A repetitions predominant anchors transforming growth factor beta on the surface of activated CD4(+)CD25(+) regulatory T cells and mediates AIDS lentivirus-induced T cell immunodeficiency: M.M. Miller, et al.; AIDS Res. Hum. Retroviruses
29, 641 (2013),
Application(s): Flow cytometry,
Abstract;
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Frequency of Circulating Regulatory T Cells Increases during Chronic HIV Infection and Is Largely Controlled by Highly Active Antiretroviral Therapy: P. Presicce, et al.; PLoS One
6, e28118 (2011),
Abstract;
Full Text
Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32): D.V. Chan, et al.; BMC
12, 27 (2011),
Abstract;
Full Text
Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells: R. Wang, et al.; PNAS
106, 13439 (2009),
Abstract;
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells: D.Q. Tran, et al.; PNAS
106, 13445 (2009),
Abstract;
Therapeutic potential of FOXP3+ regulatory T cells and their interactions with dendritic cells: D.Q. Tran & E.M. Shevach; Hum. Immunol.
70, 294 (2009),
Abstract;
General Literature References
Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression: R. Wang, et al.; PLoS ONE
3, e2705 (2008),
Abstract;
Full Text
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