Product Details
Alternative Name: | NOS (universal) |
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Clone: | NOS-3F7-B11-B5 |
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Host: | Mouse |
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Isotype: | IgM |
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Immunogen: | Purified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain. |
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UniProt ID: | E1BKI3 |
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Species reactivity: | Mouse, Rat Bovine
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Specificity: | Recognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I). |
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Applications: | IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:50-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot. |
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Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation: K.Y. Xu, et al.; J. Biol. Chem.
278, 41798 (2003),
Abstract;
Full Text
Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia: C. Xue, et al.; Am. J. Physiol.
267, L667 (1994),
Abstract;