Product Details
Alternative Name: | Acute myeloid leukemia 2 protein, Runt-related transcription factor 3, CBF-α3, AML-2, Core-binding factor α3 subunit |
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Clone: | R3-1E10 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300). |
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UniProt ID: | Q13761 |
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Species reactivity: | Human, Mouse
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 2µg/ml) Western Blot (0.2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells. |
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Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot analysis using MAb to RUNX3 (R3-1E10) (Prod. No.
ALX-803-320).
Method: 0.2µg/ml of MAb to RUNX3 (R3-1E10) were applied to the membrane on which recombinant human (lanes 1-3) or mouse (lanes 4-6) RUNX1, RUNX2 or RUNX3 expressed in COS7 cells were loaded (see [1])."
Figure 2: Immunohistochemistry using MAb to RUNX3 (R3-1E10) (Prod. No.
ALX-803-320).
Method: 2µg/ml of MAb to RUNX3 (R3-1E10) were applied to normal human gastric samples (paraffin sections) which were retrieved by autoclave at 105°C for 15 min. in Dako solution (see [1])."
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Product Literature References
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis: T. Yano, et al.; Mol. Cell. Biol.
26, 4474 (2006),
Abstract;
Full Text
General Literature References
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer: Q.C. Lau, et al.; Cancer Res.
66, 6512 (2006),
Abstract;
Full Text
Tumor suppressor activity of RUNX3: S.C. Bae & J.K. Choi; Oncogene
23, 4336 (2004),
Abstract;