Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=59,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.
Product Details
| Alternative Name: | DCDHF diacetate, 2',7'-Dichlorofluorescein diacetate |
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| Formula: | C24H16O7Cl2 |
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| MW: | 487.3 |
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| CAS: | 4091-99-0 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White to light orange-brown solid. |
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| Solubility: | Soluble in DMSO (≥25mg/ml), 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately dilute with PBS, pH 7.2, to the desired concentration. |
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| Regulatory Status: | RUO - Research Use Only |
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