Product Details
| Alternative Name: | PAR |
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| MW: | 1’100 to 275’000 depending on the number of units of ADP-ribose. |
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| Source: | Released from automodified PARP-1 by base treatment and purified by boronate affinity. |
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| Quantity: | 1µg representing 1.8 nanomoles of 2-300 units of ADP-ribose. |
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| Formulation: | Liquid. In water. |
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| Purity: | ≥95% (HPLC of ADP-ribose RNA and DNA measurement) |
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| Application Notes: | Immunoblotting standard for the detection of poly(ADP-ribose) (PAR) of 3-200 bases in size sythesized in vitro or in vivo. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for one week when stored at +4°C. Stable for at least 2 years after receipt when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Strategies Employed for the Development of PARP Inhibitors: S. Canan, et al.; Methods Mol. Biol.
1608, 271 (2017),
Abstract;
PARP activation promotes nuclear AID accumulation in lymphoma cells: S. Tepper, et al.; Oncotarget
7, 13197 (2016),
Abstract;
Full Text
Immunological determination and size characterization of poly(ADP- ribose) synthesized in vitro and in vivo: E.B. Affar, et al.; Biochim. Biophys. Acta
1428, 137 (1999),
Abstract;
Poly(ADP-ribose) turnover in quail myoblast cells: relation between the polymer level and its catabolism by glycohydrolase: E.B. Affar, et al.; Mol. Cell. Biochem.
193, 127 (1999),
Abstract;
Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo: E.B. Affar, et al.; Anal. Biochem.
259, 280 (1998),
Abstract;
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo.: G.M. Shah, et al.; Anal. Biochem.
227, 1 (1995),
Abstract;
Rapid assay of poly(ADP-ribose) glycohydrolase: L. Menard & G.G. Poirier; Biochem. Cell Biol.
65, 668 (1987),
Abstract;
