| Alternative Name: | TPO, Megakaryocyte colony stimulating factor, c-MPL ligand, MGDF |
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| MW: | ~18.7kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain). |
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| UniProt ID: | P40226 |
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| Formulation: | Lyophilized. Sterile filtered. |
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| Purity: | ≥95% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent stimulation of MO7e cells which is typically observed at concentations <1ng/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse TPO has a predicted MW of 18.7 kDa.
Figure 1: Mouse TPO Bioactivity Data. Serial dilutions of mouse TPO, starting at 200ng/ml, were added to MO7e cells growing in the presence of 1ng/ml IL-3 and 2.5ng/ml SCF. Proliferation was measure after 5 days and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse TPO was less than 0.8ng/ml. This value is comparable to the typical expected range of 1ng/ml.
