Product Details
Alternative Name: | SYT |
|
Clone: | ASV48 |
|
Host: | Mouse |
|
Isotype: | IgG2b |
|
Immunogen: | Rat brain synaptic junction protein complexes. |
|
UniProt ID: | P21707 |
|
GenBank ID: | X52772 |
|
Source: | Purified from mouse ascites. |
|
Species reactivity: | Mouse, Rat Monkey, Shark
|
|
Applications: | ICC, IHC, IP, WB
|
|
Recommended Dilutions/Conditions: | Immunocytochemistry (1:500) Western Blot (0.1µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | Detects a band of ~65kDa by Western blot. |
|
Purity Detail: | Protein G affinity purified. |
|
Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Scientific Background: | Synaptotagmins (Syt) are membrane-trafficking proteins characterized by an N-terminal transmembrane region, a variable linker, and two C-terminal C2-domains, C2A and C2B. At least twelve Synaptotagamins are expressed in vertebrates and can be found in both vesicular and plasma membranes. |
|
Regulatory Status: | RUO - Research Use Only |
|
Western blot analysis of rat brain tissue extract, probed with Synaptotagmin, mAb (ASV48).
Please mouse over
Product Literature References
Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells: A. Yamamoto, et al. ; J. Biol. Chem.
278, 3220 (2003),
Application(s): ICC using rat samples,
Abstract;
Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells: K. Mikoshiba, et al. ; J. Biol. Chem.
277, 4601 (2002),
Application(s): ICC using rat samples,
Abstract;
Synaptotagmin IV is present at the Golgi and distal parts of neurites: K. Mikoshiba, et al. ; J. Neurochem.
74, 518 (2000),
Application(s): WB using mouse samples,
Abstract;
Comparison of immunolocalization patterns for the synaptic vesicle proteins p65 and synapsin I in macaque monkey retina: A.E. Hendrickson, et al. ; Synapse
14, 268 (1993),
Application(s): ICC using monkey samples,
Abstract;
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecterory tissue: W.D. Matthew, et al.; J. Cell Biol.
91, 257 (1981),
Application(s): WB, IP using fish (shark) & rat samples,
Abstract;
Related Products