Product Details
| Alternative Name: | Neutrophil cytosolic factor 1, NCF1 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human p47Phox. |
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| UniProt ID: | P14598 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~50kDa by Western blot. |
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Originally recognized for their role in the respiratory burst of phagocytes, the NOX family of NADPH oxidases is now known to consist of at least 13 enzymes and enzyme subunits that catalyze the production of superoxide and other downstream reactive oxygen species (ROS) in nearly all cells and tissues. NOX enzymes contribute to a multitude of physiological functions including host defense, cell signaling, and differentiation, with dysregulation linked to pathological manifestations including immunosuppression, hypothyroidism, cardiovascular disease, and neurodegeneration. p47Phox, also known as neutrophil cytosolic factor 1 (NCF1), is considered the organizing subunit of NADPH oxidase enzyme complexes containing the prototypic NOX2 enzyme. Cell stimulation results in phosphorylation of p47Phox, exposing a proline rich region which mediates translocation and association with membrane-associated NOX2/p22 complexes. Translocation of p47Phox is accompanied by the p40Phox subunit and p67Phox activator subunit, which along with GTP-bound Rac completes formation of the active NOX2 oxidase complex. Defects in p47Phox are associated with chronic granulomatous disease (CGD), characterized by an inability of host macrophages and phagocytes to kill ingested microbes, leading to life-threatening infections. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of p47phox: Lane 1: MWM, Lane 2: p47Phox (human), (recombinant) (His-tag) (Prod No. ADI-OSP-550), Lane 3: RAW 264.7 Cell Lysate.
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General Literature References
The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology: K. Bedard & K.H. Krause; Physiol Rev.
87, 245 (2007),
Abstract;
Activation and assembly of the NADPH oxidase: a structural perspective: Y. Groemping & K. Rittinger; Biochem. J.
386, 401 (2005),
Abstract;
Molecular composition and regulation of the Nox family NAD(P)H oxidases: H. Sumimoto, et al.; BBRC
338, 677 (2005),
Abstract;
Assembly of the phagocyte NADPH oxidase: W.M. Nauseef; Histochem. Cell. Biol.
122, 277 (2004),
Abstract;
Autosomal recessive chronic granulomatous disease caused by defects in NCF-1, the gene encoding the phagocyte p47-phox: mutations not arising in the NCF-1 pseudogenes: D. Noack, et al.; Blood
97, 305 (2001),
Abstract;
Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat: C.M. Casimir, et al.; PNAS U.S.A.
88, 2753 (1991),
Abstract;
