| ADI-KAM-CC220-E | 100 µg | 362.00 USD |  |
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| Clone: | 3D4 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to a portion of human Topoisomerase IIα phosphorylated at Tyr1342. |
| UniProt ID: | P11388 (α isozyme), Q02880 (β isozyme) |
| Species reactivity: | Human |
| Applications: | ICC, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (10µg/ml) Western Blot (1-10µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~170kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development. |
| Regulatory Status: | RUO - Research Use Only |
![[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Western blot](https://www.enzolifesciences.com/fileadmin/files/image/KAM-CC220_WB.jpg "[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Western blot")
![[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Immunocytochemistry](https://www.enzolifesciences.com/fileadmin/files/image/KAM-CC220_ICC.jpg "[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Immunocytochemistry")
![[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Western blot](https://www.enzolifesciences.com/fileadmin/thumbs/f10bd1edcabb10c37a56fcf89c5b7e54c2fa4638-KAM-CC220_WB.jpg.jpg "[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Western blot")
![[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Immunocytochemistry](https://www.enzolifesciences.com/fileadmin/thumbs/211e4af9c8e24f5b5d800d7e95f9bb554474431e-KAM-CC220_ICC.jpg.jpg "[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4) Immunocytochemistry")
Goat anti-mouse IgG, polyclonal antibody (AP conjugate) |
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| ELISA, WB | Print as PDF | ||||||
| ADI-SAB-101-J | 1 ml | 175.00 USD | |
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Jurkat, (cell lysate) |
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| Human, WB | Print as PDF | ||||||
| ADI-LYC-JK100-F | 200 µg | 143.00 USD | |
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