Product Details
| Alternative Name: | Polo-like kinase 1 |
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| Clone: | 3F8 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant fragment from the middle of human PLK1. |
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| UniProt ID: | P53350 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Rat
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| Crossreactivity: | Weakly cross-reacts with mouse Plk1. |
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~62kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The polo-like kinases (PLKs) function in cell-cycle progression and in multiple stages of mitosis. PLK1 is activated by phosphorylation of Thr210 at the G2/M phase boundary. Active PLK1 participates in mitotic entry through activation of Cdc25C and nuclear import of cyclin B1, thereby activating the Cdc2/cyclin B kinase. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of PLK1 mAb (3F8): Lane 1: MW marker, Lane 2: HeLa, Lane 3: Jurkat, Lane 4: PC-12, Lane 5: 3T3, Lane 6: HEK-293.
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Product Literature References
Skp1-Cul1-F-box ubiquitin ligase (SCF(βTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry: A.C. Burrows, et al.; J. Biol. Chem.
287, 39021 (2012),
Application(s): Western blot,
Abstract;
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