Product Details
Alternative Name: | Cyclin-dependent kinase 1, p34cdc2, Cell division cycle 2 |
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Clone: | 5F6 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of human Cdk1 (cdc2). |
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UniProt ID: | P06493 |
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Species reactivity: | Human, Mouse, Rat Rabbit
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Applications: | IHC, IP, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (5µg/ml) Immunoprecipitation (2µg/200µl) Western Blot (1-5µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~34kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins. These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates. Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia. Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins. |
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Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of paraffin embedded section of rat testis using Cdk1/cdc2 mAb (5F6).
Immunoprecipitation analysis of Cdk1/cdc2 from A431 cells extract with normal mouse IgG (1) or Cdk1/cdc2 mAb (5F6) (2). After immunoprecipitation with the antibody, the immunocomplex was resolved by SDS-PAGE and immunoBlotted with Cdk1/cdc2 mAb (5F6). A431 crude lysate was resolved in lane 3.
Western blot analysis of human HeLa cell lysate probed with Cdk1/cdc2 mAb (5F6).
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Product Literature References
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
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