Product Details
| Alternative Name: | β-NGF |
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| MW: | ~13.6/27.2 kDa (dimer). |
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| Source: | Produced in E. coli. Contains 121/242 amino acids. |
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| UniProt ID: | P01139 |
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| Formulation: | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/µg protein (LAL test) |
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| Reconstitution: | Reconstitute in sterile water at 0.1 mg/mL. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. For long term storage, it is recommended to dilute to working aliquots in a 0.1% BSA solution. |
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| Scientific Background: | Human β-NGF is responsible for survival, differentiation and maturation of sympathetic and sensory neurons. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Murine b-NGF induced proliferation of TF-1 cells. Serial dilutions of Mouse β-NGF, starting at 100 ng/mL, were added to TF-1 cells growing in GM-CSF free media. Cell proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
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General Literature References
Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature
368, 193 (1994), (Review),
Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science
264, 772 (1994), (Review),
Abstract;
Neurotrophic factors: two are better than one : R. Nishi; Science
265, 1052 (1994), (Review),
Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J.
8, 738 (1994), (Review),
Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev.
18, 143 (1994), (Review),
Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C.R. Acad. Sci. III
316, 1158 (1993), (Review),
Abstract;
The changing scene of neurotrophic factors : H. Thoenen; TINS
14, 165 (1991), (Review),
Abstract;
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