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Streptavidin (AP Conjugate)

 
ENZ-PRT119-1000 1 ml 65.00 USD
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As part of the need to increase the sensitivity of your sandwich ELISA, this streptavidin AP conjugate can be used with your sandwich ELISA that utilizes a biotinylated detector antibody and the AMP'D® ELISA Signal Amplification kit (Prod. no. ENZ-KIT-100) which requires the presence of the AP as the conjugate.

Product Specification

Source:Native. Purified from Streptomyces avidinii (SA) and calf intestine (AP).
 
Formulation:Liquid (50 μg/ml). In 0.025M TRIS-HCl, pH 8.0, containing 0.075M sodium chloride, 0.05mM ZnSO4, 2.45mM MgCl2, 0.045% sodium azide, 0.05% BSA and 50% Stabilzyme-AP.
 
Purity Detail:Alkaline phosphatase purified from calf intestine.
 
Applications:ELISA
 
Application Notes:For certified AMP'D® ELISA kit development. The recommended concentration in an ELISA would be 500-100ng/ml, which is a 1:100 to 1:500 dilution of the sample provided.
Optimal conditions must be determined individually for each application.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

General Literature References

The critical roles of endoplasmic reticulum chaperones and unfolded protein response in tumorigenesis and anticancer therapies: B. Luo & A.S. Lee; Oncogene 32, 805 (2013), (Review), Abstract; Full Text
The critical role of GRP78 in physiologic and pathologic stress: K.T. Pfaffenbach & A.S. Lee; Curr. Opin. Cell Biol. 23, 150 (2011), (Review), Abstract; Full Text

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