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AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay Kit

Sensitive and specific qPCR assay for the detection of Ureaplasma spp. / M. genitalium / M. hominis
 
ENZ-GEN209-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 
  • Highly sensitive and specific assay
  • Low-cost alternative to other methods of Ureaplasma spp. / M. genitalium / M. hominis detection
  • Compatible with most open qPCR platforms
  • Smaller sample input that allows remaining extracted samples to be used in other tests
The AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis (UMM) Assay is a real-time polymerase chain reaction (qPCR) assay for the qualitative detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis DNA. The kit uses the AMPIPROBE® assay platform which takes advantage of paired fluorophore- and quencher-labeled primers specific for each of the target species as well as an internal control. The kit contains all reagents necessary for PCR-based detection of Ureaplasma spp., Mycoplasma genitalium, and Mycoplasma hominis DNA. A positive PCR control consisting of a mixture of the target templates and a negative PCR control that results negative for UMM, but positive for the internal control (human β-globin). Please read the complete kit insert before performing this assay.

Product Specification

Sensitivity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay was determined to have the following percentage of Sensitivity:

Ureaplasma spp. - 97.80%
M. genitalium - 87.30
M. hominis - 79.70%
 
Applications:qPCR
 
Application Notes:The AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay is compatible with any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN Rotor-Gene Q
 
Species reactivity:Ureaplasma spp., Mycoplasma genitalium, Mycoplasma hominis
 
Specificity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® Ureaplasma spp. / M. genitalium / M. hominis Assay was determined to have the following percentage of Specificity:

Ureaplasma spp. - 95.00%
M. genitalium - 100.00
M. hominis - 100.00%
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:AMPIGENE® HS Taq DNA Polymerase
AMPIGENE® dNTP Mix
AMPIPROBE® 5X Assay Buffer
AMPIPROBE® UMM Primer Mix
UMM Positive PCR Control
Negative PCR Control
Nuclease-free Water
 
Scientific Background:Ureaplasma and Mycoplasma, which belong to the same Mycoplasmataceae family and Mollicutes class, are the smallest self-replicating organisms and are characterized by their lack of a cell wall. These characteristics and limited biosynthetic capabilities contribute to the parasitic nature of Ureaplasma and Mycoplasma.
The class of pathogens is present in healthy individuals but has been associated with many adverse conditions affecting the reproductive tract. Mycoplasma genitalium is associated with urethritis, cervical inflammation, and pelvic inflammatory disease. M. hominis is often present concurrently with Ureaplasma species and is associated with a variety of conditions ranging from pelvic inflammatory diseases, chorioamnionitis, postpartum endometritis bacterial vaginosis, arthritis, osteoarthritis, wound infections, and several conditions in neonates.
Ureaplasma and Mycoplasma, especially in combination with other conditions such as bacterial vaginosis or cervical incompetence, have been associated with adverse pregnancy outcomes, such as chorioamnionitis, spontaneous preterm labor and preterm premature rupture of membranes.
The AMPIPROBE® UMM Assay provides rapid and accurate results for the qualitative detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis DNA in a user-supplied sample of interest.
 

General Literature References

Ureaplasma urealyticum, Mycoplasma hominis and adverse pregnancy outcomes: R. Capoccia, et al.; Curr. Opin. Infect. Dis. 26, 231 (2013), Abstract;
Molecular Methods for the Detection of Mycoplasma and Ureaplasma Infections in Humans: K.B. Waites, et al.; J. Mol. Diagn. 5, 437 (2012), Abstract; Full Text
Mycoplasmas and ureaplasmas as neonatal pathogens: K.B. Waites, et al.; Clin. Microbiol. Rev. 18, 757 (2005), Abstract; Full Text
Mycoplasma genitalium: the aetiological agent of urethritis and other sexually transmitted diseases: J.S. Jensen; J. Eur. Acad. Dermatol. Venereol. 18, 1 (2004), Abstract;
Molecular Biology and Pathogenicity of Mycoplasmas: S. Razin, et al.; Microbiol. Mol. Biol. Rev. 4, 1094 (1998), Abstract; Full Text

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