| Alternative Name: | Solute carrier family 2 facilitated glucose transporter member 1, Glucose transporter type 1 (erythrocyte/brain), HEPG2 glucose transporter |
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| Clone: | 2475 |
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| Host: | Mouse |
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| Isotype: | IgG2Bκ |
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| Immunogen: | A portion of amino acids 203-305 from the human protein was used as the immunogen for this GLUT1 antibody. |
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| UniProt ID: | P11166 |
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| Species reactivity: | Human
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| Specificity: | Recognizes a protein of 45-55kDa, which is identified as GLUT1. |
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| Applications: | ELISA, Flow Cytometry, IF, IHC, WB
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| Purity Detail: | Protein G affinity purified |
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| Formulation: | Liquid. In 1X PBS containing 0.1 mg/ml BSA and 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative). |
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| Regulatory Status: | RUO - Research Use Only |
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Immunofluorescent staining of human K562 cells with GUT1 antibody (green) and Reddot nuclear stain (red).
IHC testing of human bladder carcinoma with GLUT1 antibody (clone GLUT1/2475). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of human tongue with GLUT1 antibody (clone GLUT1/2475). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified GLUT1 antibody.
