| Alternative Name: | CD366, TIMD3 |
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| Clone: | 1B10 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Extracellular domain of human TIM-3. |
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| UniProt ID: | Q8TDQ0 |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry, ICC, IF, IHC (PS), WB
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide and 50% glycerol. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells. TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice. Blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 µg/mL.
Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 µg/mL. Green: TIM-3 Antibody [1B10] Blue: DAPI staining
Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 µg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 µg/mL.
Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 µg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.
