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V5-tag monoclonal antibody (E10/V4RR) (HRP conjugate)

 
ENZ-ABS287-0050 50 µl 147.00 USD
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Product Specification

Clone:E10/V4RR
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to GKPIPNPLLGLDST (V5), conjugated to KLH.
 
Species reactivity:Species independent
 
Applications:WB
 
Purity Detail:Purified
 
Formulation:Liquid. In PBS containing a proprietary stabilizer and no preservative.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the epitope tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered protein, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
 
ENZ-ABS287 WB
Figure 1. Western Blot with V5-tag, mAb (E10/V4RR) (HRP conjugate ) (Prod. no ENZ-ABS287)
Western blot analysis of V5 Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with V5-tag, mAb (E10/V4RR) (HRP conjugate ) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed.
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ENZ-ABS287 WB

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