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Propidium Iodide (ultra pure)

DNA dye
 
ENZ-52403 100 mg 153.00 USD
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Replaces Prod. #: BML-AP401

Propidium iodide (PI) belongs to the same chemical class as ethidium bromide. As with ethidium bromide, its fluorescence is enhanced 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted 30-40 nm and the fluorescence emission maximum blueshifted ~15 nm. PI also binds to RNA as does DAPI and acridine orange. PI is membrane impermeant and is commonly used for identifying dead cells in a mixed population of cells and as a counter-stain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and viable cells. The dye is suitable for fluorescence microscopy, flow cytometry and fluorometry applications. Wavelength Maxima: Excitation 535nm, Emission 617nm

Product Specification

Formula:C27H34I2N4
 
MW:668.4
 
CAS:25535-16-4
 
Purity:≥95% (HPLC)
 
Appearance:Dark red solid.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 
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Product Literature References

The novel protective role of P27 in MLN4924-treated gastric cancer cells: Q. Zhang, et al.; Cell. Death Dis. 6, e1867 (2015), Application(s): Viability staining, Abstract; Full Text
High-throughput cytotoxicity screening by propidium iodide staining: B.S. Edwards, et al.; Curr. Protoc. Cytom. Chapter 9, Unit9 24 (2007), Abstract;

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