Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

ROS-ID™ Superoxide detection kit

 
ENZ-51012 1 Kit 169.00 USD
Do you need bulk/larger quantities?
 
  • Directly monitors global levels of  superoxide in live cells by fluorescence microscopy or flow cytometry
  • High sensitivity, specificity and accuracy for live cell studies
  • Compatible with major components of tissue culture media (phenol red, FBS and BSA)
  • Complete set of reagents, including ROS inducer and scavenger
Enzo Life Sciences’ ROS-ID™ Superoxide detection kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with a standard orange (e.g., 550/620 nm) fluorescent cube, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. The Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).

Product Specification

Applications:FC, Fluorescence microscopy, Fluorescent detection, HTS
 
Application Notes:This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.
 
Quality Control:A sample from each lot of ROS-ID™ Superoxide detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard orange (e.g., 550/620 nm) fluorescent cube.
The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright orange fluorescence in the nucleus. Cells pretreated with the ROS inhibitor don’t demonstrate any orange fluorescence signal upon induction.
 
Quantity:200 fluorescence microscopy assays or 50 flow cytometry assays.
 
Use/Stability:With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Kit/Set Contains:Superoxide Detection Reagent (Orange), 300 nmoles
ROS Inducer (Pyocyanin), 1 µmole
ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg
Wash Buffer Salts, 1 pack
 
Technical Info/Product Notes:The ROS-ID™ Superoxide detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

Product Literature References

Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression: L. Pasterk, et al.; Sci. Rep. 6, 22104 (2016), Application(s): Intracellular ROS production, Abstract;
Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells: N. Shin, et al.; Biochem. Biophys. Res. Commun. 459, 699 (2015), Application(s): Flow Cytometry, Abstract;

General Literature References

Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects: P. Wardman; Free Radic. Biol. Med. 43, 995 (2007), Abstract;
Fluorescence probes used for detection of reactive oxygen species: A. Gomes, et al.; J. Biochem. Biophys. Methods 65, 45 (2005), Abstract;
Determination of mitochondrial reactive oxygen species: methodological aspects: C. Batandier, et al.; J. Cell. Mol. Med. 6, 175 (2002), Abstract;
Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite: M.M. Tarpey & I. Fridovich; Circ. Res. 89, 224 (2001), Abstract;

Related Products

ROS-ID™ Total ROS/Superoxide detection kit 

Directly Monitor Global Levels of Reactive Oxygen Species (ROS), and Specifically Superoxide
FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51010 1 Kit 230.00 USD
Do you need bulk/larger quantities?
 

ROS-ID™ Total ROS detection kit 

FC, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51011 1 Kit 169.00 USD
Do you need bulk/larger quantities?
 

ROS-ID™ ROS/RNS detection kit 

Monitor reactive oxygen species and nitrogen species in live cells
Fluorescence microscopy | Print as PDF
 
ENZ-51001-200 1 Kit 275.00 USD
Do you need bulk/larger quantities?
 

ROS-ID™ NO Detection kit 

Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51013-200 1 Kit 169.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Visualize Cellular Responses with CELLESTIAL® Fluorescent Probes
Visualize Cellular Responses with CELLESTIAL® Fluorescent Probes
Download as PDF

Brochures
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Download as PDF

Brochures
Predictive Toxicology
Predictive Toxicology
Download as PDF

Product Flyers
New Tools for Flow Cytometry
New Tools for Flow Cytometry
Download as PDF

Scientific Posters
Disease-Associated Stress Signaling
Disease-Associated Stress Signaling
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2016 Enzo Life Sciences, Inc.,