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Superoxide detection kit for microscopy and flow cytometry

 
ENZ-51012 1 Kit 160.00 USD
 
  • Directly monitors global levels of  superoxide in live cells by fluorescence microscopy or flow cytometry
  • High sensitivity, specificity and accuracy for live cell studies
  • Compatible with major components of tissue culture media (phenol red, FBS and BSA)
  • Complete set of reagents, including ROS inducer and scavenger
Enzo Life Sciences’ Superoxide Detection Kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard orange (e.g., 550/620 nm) or red (e.g., 650/670 nm) fluorescent cubes, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. The Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).

Product Specification

Application:This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.
 
Quality Control:A sample from each lot of Superoxide Detection Kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard orange (e.g., 550/620 nm) fluorescent cube. The following results are obtained: - ROS positive control samples induced with Pyocyanin exhibit bright orange fluorescence in the nucleus. - Cells pretreated with the ROS inhibitor don’t demonstrate any orange fluorescence signal upon induction.
 
Quantity:200 fluorescence microscopy assays or 50 flow cytometry assays.
 
Use/Stability:With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Kit/Set Contains:Superoxide Detection Reagent (Orange), 300 nmoles
ROS Inducer (Pyocyanin), 1 µmole
ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg 
Wash Buffer Salts, 1 pack
 
Background / Technical Information:The Superoxide detection kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
 

General Literature References

Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects: P. Wardman; Free Radic. Biol. Med. 43, 995 (2007), Abstract;
Fluorescence probes used for detection of reactive oxygen species: A. Gomes, et al.; J. Biochem. Biophys. Methods 65, 45 (2005), Abstract;
Determination of mitochondrial reactive oxygen species: methodological aspects: C. Batandier, et al.; J. Cell. Mol. Med. 6, 175 (2002), Abstract;
Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite: M.M. Tarpey & I. Fridovich; Circ. Res. 89, 224 (2001), Abstract;

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