Total ROS detection kit for microscopy and flow cytometry



ENZ-51011	1 Kit	148.00 USD

Directly monitors global levels of reactive oxygen species (ROS), but not superoxide in live cells
Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals when specific inhibitors are used
High sensitivity, specificity and accuracy for live cell studies
Compatible with major components of tissue culture media (phenol red, FBS and BSA)
Complete set of reagents, including ROS inducers and scavengers
Stringently manufactured, to control and eliminate non-specific assay artifacts

Enzo Life Sciences’ Total ROS Detection Kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry. The kit includes Oxidative Stress Detection Reagent (Green) as the major component. This non-fluorescent, cell-permeable total ROS detection dye reacts directly with a wide range of reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals, yielding a green fluorescent product indicative of cellular production of different ROS/RNS types.

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Product Specification

Alternative Name:	Reactive Oxygen Species detection kit

Quantity:	200 fluorescence microscopy assays or 50 flow cytometry assays.

Quality Control:	A sample from each lot of Total ROS Detection Kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard green filter (490/525 nm). The following results are obtained: - ROS positive control samples induced with Pyocyanin exhibit bright green fluorescence in the cytoplasm. - Cells pretreated with the ROS inhibitor don’t demonstrate any green fluorescence signal upon induction.

Kit/Set Contains:	Oxidative Stress Detection Reagent (Green), 300 nmoles ROS Inducer (Pyocyanin), 1 µmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack

Application:	This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

Short Term Storage:	-20°C

Long Term Storage:	-80°C

Use/Stability:	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	The Total ROS Detection Kit is a member of the CELLestial^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Product Literature References

Deoxycholic acid causes DNA damage while inducing apoptotic resistance through NF-{kappa}B activation in benign Barrett's epithelial cells: X. Huo, et al.; Am J Physiol Cell Physiol Jun 2, 278 (2011), Abstract;

Depletion of cytosolic or mitochondrial thioredoxin increases CYP2E1-induced oxidative stress via an ASK-1-JNK1 pathway in HepG2 cells: L. Wang, et al.; Free Radic Biol Med 1;51(1), 185-96. (2011), Abstract;

Enhancement of the radiation effects by D-allose in head and neck cancer cells: H. Hoshikawa, et al.; Cancer Lett. 306(1), 60 (2011), Abstract;

Formation of TiO2 Nanostructures by Enzyme-Mediated Self-Assembly for the Destruction of Macrophages : K. Hayashi, et al.; Chem of Materials 23 (14), 3341 (2011), Abstract;

Protective effects of cynaroside against H2O2-induced apoptosis in H9c2 cardiomyoblasts: X. Sun, et al.; J Cell Biol 112(8), 2019 (2011), Abstract;

CYP2E1 enhances ethanol-induced lipid accumulation but impairs autophagy in HepG2 E47 cells: D. Wu, et al; Biochem Biophys Res Commun 5;402(1), 116 (2010), Abstract;

General Literature References

Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects: P. Wardman; Free Radic. Biol. Med. 43, 995 (2007), Abstract;

Fluorescence probes used for detection of reactive oxygen species: A. Gomes, et al.; J. Biochem. Biophys. Methods 65, 45 (2005), Abstract;

Determination of mitochondrial reactive oxygen species: methodological aspects: C. Batandier, et al.; J. Cell. Mol. Med. 6, 175 (2002), Abstract;

Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite: M.M. Tarpey & I. Fridovich; Circ. Res. 89, 224 (2001), Abstract;