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Bio-16-UTP

 
ENZ-42814 250 nmol 250.00 USD
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Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.

Product Specification

Alternative Name:Biotin-16-uridine-5’-triphosphate
 
Formula:C32H52N7O19P3S
 
MW:963.7 (free acid)
 
Concentration:10mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:10,700 M-1 cm-1 (240 nm, pH 7.0)
 
Purity:≥93% (HPLC)
 
Purity Detail:Contains <5% Bio-16-UDP.
 
Appearance:Clear, colorless liquid.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

 
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Product Literature References

Whole-genome transcription and DNA methylation analysis of peripheral blood mononuclear cells identified aberrant gene regulation pathways in systemic lupus erythematosus: H. Zhu, et al.; Arthritis Res. Ther. 18, 162 (2016), Application(s): Gene expression studies, RNA samples, Abstract; Full Text

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