Bio-N6-ddATP (Biotin-N6-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N6-ddAMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes. Biotin-labeled cDNA produced by this reaction can be used in nucleic acid array assays. Biotin-labeled DNA in hybridization assays can be detected by a reporter molecule linked to streptavidin, avidin, or and anti-biotin antibody. Such a complex can be detected directly, e.g. by the excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate. Bio-N6-ddATP is also a substrate for Taq DNA Polymerase and other DNA polymerases.
Product Details
| Alternative Name: | Biotin-N6-(2’,3’-dideoxy)-adenosine-5’-triphosphate |
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| Formula: | C36H61N10O15P3S |
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| MW: | 998.9 (free acid) |
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| Concentration: | 1mM |
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| Formulation: | Liquid. Solution in water. |
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| Extinction Coefficient: | 17,000 M-1 cm-1 (265 nm, pH 7.0) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Contains <5% Bio-N6-ddADP. |
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| Appearance: | Clear, colorless liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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