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Bio-N6-ddATP

 
ENZ-42809 25 nmol 254.00 USD
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Bio-N6-ddATP (Biotin-N6-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N6-ddAMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes. Biotin-labeled cDNA produced by this reaction can be used in nucleic acid array assays. Biotin-labeled DNA in hybridization assays can be detected by a reporter molecule linked to streptavidin, avidin, or and anti-biotin antibody. Such a complex can be detected directly, e.g. by the excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate. Bio-N6-ddATP is also a substrate for Taq DNA Polymerase and other DNA polymerases.

Product Specification

Alternative Name:Biotin-N6-(2’,3’-dideoxy)-adenosine-5’-triphosphate
 
Formula:C36H61N10O15P3S
 
MW:998.9 (free acid)
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:17,000 M-1 cm-1 (265 nm, pH 7.0)
 
Purity:≥93% (HPLC)
 
Purity Detail:Contains <5% Bio-N6-ddADP.
 
Appearance:Clear, colorless liquid.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
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