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Cyanine 5-dUTP

 
ENZ-42502 25 nmol 175.00 USD
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Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Product Specification

Alternative Name:Cyanine 5-deoxyuridine-5’-triphosphate
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Absorbance maximum:650 nm
 
Emission maximum:662 nm
 
Extinction Coefficient:250,000 M-1 cm-1 (650 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Blue liquid.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
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Product Literature References

Amplification-free sequencing of cell-free DNA for prenatal non-invasive diagnosis of chromosomal aberrations: K. Karlsson, et al.; Genomics 105, 150 (2015), Application(s): Oligonucleotide Array, Abstract; Full Text
Mitochondria-focused gene expression profile reveals common pathways and CPT1B dysregulation in both rodent stress model and human subjects with PTSD: L. Zhang, et al.; Transl. Psychiatry 5, e580 (2015), Application(s): Labeling of cDNA, Abstract; Full Text
Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers: Y. Lv, et al.; Nat. Protoc. 10, 1508 (2015), Application(s): Labeling of cDNA, Abstract;
Gestational nicotine treatment modulates cell death/survival-related pathways in the brains of adolescent female rats: J. Wie, et al.; Int. J. Neuropsychopharmacol. 14, 91 (2011), Abstract;
Modulation of cell adhesion systems by prenatal nicotine exposure in limbic brain regions of adolescent female rats: J. Cao, et al.; Int. J. Neuropsychopharmacol. 14, 157 (2011), Abstract;

General Literature References

DNA Microarrays: A Molecular Cloning Manual: D. Bowtell & J Sambrook (eds.); Cold Spring Harbor Laboratory Press (2003), Abstract;
DNA arrays for analysis of gene expression: M.B. Eisen & P.O. Brown; Methods Enzymol. 303, 179 (1999), Abstract;
A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization: D. Shalon, et al.; Genome Res. 6, 639 (1996), Abstract;

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