Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
Product Details
| Alternative Name: | Cyanine 5-deoxyuridine-5’-triphosphate |
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| Concentration: | 1mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 650 nm |
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| Emission maximum: | 662 nm |
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| Extinction Coefficient: | 250,000 M-1 cm-1 (650 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Blue liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Application(s): Labeling of cDNA,
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Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers: Y. Lv, et al.; Nat. Protoc.
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Application(s): Labeling of cDNA,
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