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SUMO activating enzyme E1 (human), (recombinant)

 
BML-UW9330-0025 25 µg 261.00 USD
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Product Specification

MW:~38kDa and 90kDa
 
Source:Produced in E. coli. Full length heterodimeric human SUMO-activating enzyme.
 
UniProt ID:Q9UBE0 (subunit 1), O95605 (subunit 2)
 
Formulation:Liquid. In HEPES, pH 8.0, containing 110mM potassium acetate, 2mM magnesium acetate, 0.5mM EGTA and 1mM DTT.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. SUMO activating enzyme is a heterodimeric complex consisting of Aos1 and Uba2. Both subunits are well conserved from yeast to humans, with Aos2/SAE I being similar to the N-terminal half of the E1 enzyme for ubiquitin while Uba2/SAE II has similarity to the C-terminal half, and contains the active site cysteine residue required for formation of thioester bonds. However, Uba2 alone is not sufficient to catalyze SUMOylation. SUMO1 activating enzyme supports in vitro SUMOylation.
 
BML-UW9330 SDS-PAGE1
SUMO E1 heterodimer by SDS-PAGE. Lane M: Markers, in kDa (from top to bottom), 205, 116, 97, 84, 66, 55, 45 and 36; Lane 1: SUMO E1 heterodimer, final product solution.
BML-UW9330 SDS-PAGE2
SUMOylation assay utilising SUMO-1, SUMO E1 (Prod. No. BML-UW9330), SUMO E2 (Prod. No. BML-UW9320) and RanGAP1 as target protein in presence (lane 2) and absence (lanes (1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with Prod. No. BML-PW9460 (Anti-SUMO1).
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BML-UW9330 SDS-PAGE1 BML-UW9330 SDS-PAGE2

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