UbcH12 (human), (recombinant) (His-tag)

Source/Host:	Produced in E. coli.

Application:	NEDD8 modification of specific proteins in vitro. Demonstrate novel proteins are potential targets for NEDDylation under in vitro conditions. Generate substrates for deNEDDylating enzymes. NEDDylation pathway studies

Long Term Storage:	-80°C

Miscellaneous/General:	UbcH12, the major NEDD8 E2 enzyme, conjugates activated NEDD8 and mediates its subsequent linkage, via C-terminal isopeptide bond formation, to a number of proteins, including the tumour suppressors p53 and VHL, in addition to its well characterised modification of members of the cullin ubiquitin E3 ligase family. Such modifications allow NEDD8 to play a critical regulatory role in cell proliferation and development, a process closely associated to the ubiquitin-proteasome pathway.

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