Product Details
| MW: | 8565 Da |
| |
| Source: | Produced in E. coli. |
| |
| CAS: | 79586-22-4 |
| |
| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
| |
| Formulation: | Lyophilized. |
| |
| Purity: | ≥98% (SDS-PAGE) |
| |
| Appearance: | White powder. |
| |
| Solubility: | Soluble in water |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Ubiquitin is a 76 amino acid protein,which when covalently linked to proteins as a branched chain, serves as a tag marking proteins for a variety of cellular activities. Its primary sequence is remarkably conserved, with only three amino acid changes between the yeast and human sequences. Originally isolated from thymus and assumed to be a thymic hormone its primary role is now recognised as being in ATP-dependent protein degradation. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Protocol to detect in vitro and in cell ubiquitylation of mitochondrial DNA polymerase gamma by mitochondrial E3 ligase MITOL: M. Hussein, et al.; STAR Protoc.
3, 101710 (2022),
Abstract;
Biophysical and biological evaluation of optimized stapled peptide inhibitors of the linear ubiquitin chain assembly complex (LUBAC): F. Aguilar-Alonso, et al.; Bioorg. Med. Chem.
26, 1179 (2018),
Abstract;
BLM Potentiates c-Jun Degradation and Alters Its Function as an Oncogenic Transcription Factor: R. Priyadarshini, et al.; Cell Rep.
24, 947 (2018),
Abstract;
The Monocot-Specific Receptor-like Kinase SDS2 Controls Cell Death and Immunity in Rice: J. Fan, et al; Cell Host Microbe
23, 498 (2018),
Abstract;
Full Text
BubR1 Promotes Bub3-Dependent APC/C Inhibition during Spindle Assembly Checkpoint Signaling: K. Overlack, et al.; Curr. Biol.
27, 2915 (2017),
Abstract;
Full Text
Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability: S.S. Kharat, et al.; Oncogene
35, 125 (2016),
Application(s): In vitro ubiquitylation,
Abstract;
F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7: Y. Liu, et al.; Cell Death Dis.
6, e1630 (2015),
Application(s): Assay,
Abstract;
Full Text
Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF182: S.M. Pedersen, et al.; Mol. Cell. Biol.
36, 794 (2015),
Abstract;
Full Text
Pro-apoptotic F-box protein Fbxl7 regulates mitochondrial function by mediating the ubiquitylation and proteasomal degradation of survivin: Y. Liu, et al.; J. Biol. Chem.
290, 11843 (2015),
Application(s): Assay,
Abstract;
Full Text
The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy: D.A. Tumbarello, et al.; PLoS One
11, e1005174 (2015),
Application(s): Western Blot,
Abstract;
Full Text
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol.
97, 518 (2015),
Application(s): Western Blot,
Abstract;
Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS): J.E. Gilda, et al.; PLoS One
10, e0135392 (2015),
Abstract;
Full Text
The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR: L. Zhou, et al.; PLoS One
6, e23936 (2011),
Abstract;
Full Text
The ubiquitin system: A. Hershko et al.; Annu. Rev. Biochem.
67, 425 (1998),
Abstract;
Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells: G. Goldestein et al.; PNAS
72, 11 (1975),
Abstract;
